Key features and details
- Rabbit polyclonal to CFA
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CFA antibody
See all CFA primary antibodies
DescriptionRabbit polyclonal to CFA
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Cow
Synthetic peptide within Human CFA aa 58-108. The exact sequence is proprietary.
Database link: O75347
- WB: HeLa, HEK-293T and jurkat whole cell lysate. IP: CFA IP in HEK-293T whole cell lysate.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntibody was affinity purified using an epitope specific to CFA immobilized on solid support.
Our Abpromise guarantee covers the use of ab242047 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 13 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionTubulin-folding protein; involved in the early step of the tubulin folding pathway.
Sequence similaritiesBelongs to the TBCA family.
Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
- CFA antibody
- Chaperonin cofactor A antibody
- Co chaperonin associated with a & b tubulin antibody
All lanes : Anti-CFA antibody (ab242047) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 15 µg per lane.
Developed using the ECL technique.
Predicted band size: 13 kDa
Exposure time: 75 seconds
Lysates prepared in NETN buffer.
CFA was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab242047 at 6 µg per reaction. Western blot was performed on the immunoprecipitate using ab242047 at 1 µg/mL.
Lane 1: ab242047 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG in HEK-293T whole cell lysate.
Detection: Chemiluminescence with an exposure time of 30 seconds.
Lysates prepared in NETN buffer.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab242047 has not yet been referenced specifically in any publications.