Overview

  • Product name

    Anti-CFP10 antibody
  • Description

    Rabbit polyclonal to CFP10
  • Host species

    Rabbit
  • Specificity

    ab45074 recognises native and E coli expressed recombinant CFP10.
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Full length protein (Mycobacterium tuberculosis)

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    Purity: > 95% (by SDS-PAGE)
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab45074 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: Use at a concentration of 0.2 - 1 µg/ml. Detects a band of approximately 12.3 kDa.
    Application note: Reducing conditions.



    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance

      CFP10 (10 kDa culture filtrate protein) is a low molecular weight protein isolated from Mycobacterium tuberculosis culture filtrates. CFP10 and the 6 kDa early secretory antigen of T cells (ESAT6) are secreted in abundance by Mycobacterium tuberculosis and are frequently recognized by T cells from infected people. The genes encoding these proteins have been deleted from the genome of the vaccine strain Bacille Calmette Guerin (BCG). CFP10 is one of a small number of secreted M. tuberculosis proteins now identified, that appear to play key roles in tuberculosis pathogenesis and in the stimulation of immunity.
    • Cellular localization

      Secreted
    • Alternative names

      • 10 kDa culture filtrate antigen cfp10 antibody
      • Culture filtrate protein 10 antibody
      • ESAT 6 like protein esxB antibody
      • esxB antibody
      • lhp antibody
      • mtsA10 antibody
      • Secreted antigenic protein MTSA 10 antibody
      • tuberculosis CFP10 antibody
      see all

    References

    This product has been referenced in:

    • Lai LY  et al. Role of the Mycobacterium marinum ESX-1 Secretion System in Sliding Motility and Biofilm Formation. Front Microbiol 9:1160 (2018). Read more (PubMed: 29899738) »
    • Lou Y  et al. EspC forms a filamentous structure in the cell envelope of Mycobacterium tuberculosis and impacts ESX-1 secretion. Mol Microbiol 103:26-38 (2017). Read more (PubMed: 27859904) »
    See all 4 Publications for this product

    Customer reviews and Q&As

    1-4 of 4 Abreviews or Q&A

    Abreviews
    Application
    ELISA
    Sample
    Mycobacterium tuberculosis Recombinant protein (ESAT6-CFP10)
    Specification
    ESAT6-CFP10
    Blocking step
    Milk as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Type
    Sandwich (Capture)

    Abcam user community

    Verified customer

    Submitted Feb 13 2018

    Application
    Immunoprecipitation
    Sample
    Mycobacterium tuberculosis Cell lysate - other (M. tuberculosis culture filtrate)
    Total protein in input
    1000 µg
    Specification
    M. tuberculosis culture filtrate
    Immuno-precipitation step
    Protein G

    Abcam user community

    Verified customer

    Submitted Jul 11 2012

    Application
    Western blot
    Sample
    Mycobacterium tuberculosis Purified protein (M. tuberculosis culture filtrate)
    Loading amount
    10 µg
    Specification
    M. tuberculosis culture filtrate
    Gel Running Conditions
    Reduced Denaturing (15% SDS-PAGE)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5µg/mL · Temperature: 22°C

    Abcam user community

    Verified customer

    Submitted Jun 27 2012

    Answer

    Thank you for your interest in Abcam.

    1) Indeed, I can confirm that all relevant antibodies (CFP10: ab45074, ab64753, ab64754; ESAT6: ab26246, ab45073; Ag85B: ab43019) have been generated against the full-length proteins. The exact binding sequences of these have unfortunately not been determined, except for ab26246 (residues EQQWNFAGIEAAA at positions 3-15). So if you are working with protein fragments (or epitopes), we cannot guarantee that these antibodies will work for you, as the binding sites might not be present and therefore the antibody might not bind. I could ask the lab whether the epitopes have been mapped and whether the antibodies would bind your fragments, if you could provide some details to your peptides.

    2) We continually add antibodies and other protein detection products to our catalogue but we do not offer custom-made antibodies. However, we are happy to pass on the contact details of the following companies that do offer this service:

    United States

    Pocono Rabbit Farm and Laboratory (PRFL)
    PO Box 240
    Canadensis, PA 18325
    Tel: 800-622-6381 - Toll Free in the United Statesand Canada
    Tel: 570-595-7108 - Worldwide
    Fax: 570-595-9277
    Website: www.prfal.com

    Maine Biotechnology Services, Inc.
    1037R Forest Ave.
    Portland Maine 04103
    Tel: 800-925-9476 or 207-797-5454
    Fax: 207-797-5595
    Website: www.mainebiotechnology.com

    Cocalico Biologicals, Inc.
    PO Box 265
    449 Stevens Road
    Reamstown, PA 17567-0265
    Tel: 717-336-1990
    Fax: 717-336-1993
    Website: www.cocalicobiologicals.com

    United Kingdom

    Charles River Laboratories International, Inc.
    Tel: +44 (0) 1843 823575
    Website: www.criver.com

    I hope this information is helpful to you. Please do not hesitate to contact us if you have any questions.

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