Anti-CFP10 antibody (ab45074)
Key features and details
- Rabbit polyclonal to CFP10
- Suitable for: WB
- Reacts with: Species independent
- Isotype: IgG
Overview
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Product name
Anti-CFP10 antibody -
Description
Rabbit polyclonal to CFP10 -
Host species
Rabbit -
Specificity
ab45074 recognises native and E coli expressed recombinant CFP10. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Recombinant full length protein corresponding to CFP10.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Purity: > 95% (by SDS-PAGE) -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab45074 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
Use at an assay dependent concentration.
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| Notes |
|---|
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WB
Use at an assay dependent concentration. |
Target
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Relevance
CFP10 (10 kDa culture filtrate protein) is a low molecular weight protein isolated from Mycobacterium tuberculosis culture filtrates. CFP10 and the 6 kDa early secretory antigen of T cells (ESAT6) are secreted in abundance by Mycobacterium tuberculosis and are frequently recognized by T cells from infected people. The genes encoding these proteins have been deleted from the genome of the vaccine strain Bacille Calmette Guerin (BCG). CFP10 is one of a small number of secreted M. tuberculosis proteins now identified, that appear to play key roles in tuberculosis pathogenesis and in the stimulation of immunity. -
Cellular localization
Secreted -
Alternative names
- 10 kDa culture filtrate antigen cfp10 antibody
- Culture filtrate protein 10 antibody
- ESAT 6 like protein esxB antibody
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Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab45074 has been referenced in 5 publications.
- Soler-Arnedo P et al. Polarly Localized EccE1 Is Required for ESX-1 Function and Stabilization of ESX-1 Membrane Proteins in Mycobacterium tuberculosis. J Bacteriol 202:N/A (2020). PubMed: 31843799
- Lai LY et al. Role of the Mycobacterium marinum ESX-1 Secretion System in Sliding Motility and Biofilm Formation. Front Microbiol 9:1160 (2018). PubMed: 29899738
- Lou Y et al. EspC forms a filamentous structure in the cell envelope of Mycobacterium tuberculosis and impacts ESX-1 secretion. Mol Microbiol 103:26-38 (2017). PubMed: 27859904
- Sharma G et al. The interaction of mycobacterial protein Rv2966c with host chromatin is mediated through non-CpG methylation and histone H3/H4 binding. Nucleic Acids Res 43:3922-37 (2015). WB ; Human . PubMed: 25824946
- Ramsdell TL et al. Linked domain architectures allow for specialization of function in the FtsK/SpoIIIE ATPases of ESX secretion systems. J Mol Biol 427:1119-32 (2015). PubMed: 24979678