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Anti-CFTR antibody (ab2916)

  • Datasheet
  • SDS
Reviews (1)Q&A (4)References (3)

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Immunocytochemistry/ Immunofluorescence - Anti-CFTR antibody (ab2916)

    Key features and details

    • Rabbit polyclonal to CFTR
    • Suitable for: ICC/IF
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Protein
    Product image
    Recombinant Human CFTR protein (ab114246)
    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-CFTR antibody
      See all CFTR primary antibodies
    • Description

      Rabbit polyclonal to CFTR
    • Host species

      Rabbit
    • Specificity

      Detects cystic fibrosis transmembrane conductance factor (CFTR) from cells overexpressing the human protein.
    • Tested Applications & Species

      Application Species
      ICC/IF
      Human
      See all applications and species data
    • Immunogen

      Synthetic peptide corresponding to Human CFTR aa 103-117.
      Sequence:

      GRIIASYDPDNKEER

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      Preservative: 0.05% Sodium azide
      Constituents: 99% PBS, 0.1% BSA
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Primary antibody notes

      Cystic Fibrosis (CF) is a common lethal genetic disease caused by mutations of the gene coding for the cystic fibrosis transmembrane conductance factor, a cAMP regulated chloride channel. Approximately 70% of all CF cases share the deletion of a phenylalanine at position 508 (delta F508) which results in abnormal chloride transport. Since the CF mutation is lethal, most often by lung and liver disease, it raises the question of why this genetic disease remains as common as it is. One possible explanation is that Salmonella typhi has been shown to use CFTR to enter intestinal epithelial cells and that delta F508 heterozygote and homozygote mice showed 86% and 100% reductions in S. typhi intestinal submucosal uptake.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Cell Biology
      • Other Antibodies
      • Other Antibodies
      • Metabolism
      • Types of disease
      • Cancer

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human CFTR protein (ab114246)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab2916 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    ICC/IF
    Human
    All applications
    Rat
    Sheep
    Rabbit
    Guinea pig
    Cow
    Dog
    Pig
    Chimpanzee
    Monkey
    Non human primates
    Cynomolgus monkey
    Rhesus monkey
    Gorilla
    Elephant
    Application Abreviews Notes
    ICC/IF
    Use a concentration of 1 µg/ml.
    Notes
    ICC/IF
    Use a concentration of 1 µg/ml.

    Target

    • Function

      Involved in the transport of chloride ions. May regulate bicarbonate secretion and salvage in epithelial cells by regulating the SLC4A7 transporter.
    • Tissue specificity

      Found on the surface of the epithelial cells that line the lungs and other organs.
    • Involvement in disease

      Defects in CFTR are the cause of cystic fibrosis (CF) [MIM:219700]; also known as mucoviscidosis. CF is the most common genetic disease in the Caucasian population, with a prevalence of about 1 in 2'000 live births. Inheritance is autosomal recessive. CF is a common generalized disorder of exocrine gland function which impairs clearance of secretions in a variety of organs. It is characterized by the triad of chronic bronchopulmonary disease (with recurrent respiratory infections), pancreatic insufficiency (which leads to malabsorption and growth retardation) and elevated sweat electrolytes.
      Defects in CFTR are the cause of congenital bilateral absence of the vas deferens (CBAVD) [MIM:277180]. CBAVD is an important cause of sterility in men and could represent an incomplete form of cystic fibrosis, as the majority of men suffering from cystic fibrosis lack the vas deferens.
    • Sequence similarities

      Belongs to the ABC transporter superfamily. ABCC family. CFTR transporter (TC 3.A.1.202) subfamily.
      Contains 2 ABC transmembrane type-1 domains.
      Contains 2 ABC transporter domains.
    • Domain

      The PDZ-binding motif mediates interactions with GOPC and with the SLC4A7, SLC9A3R1/EBP50 complex.
    • Post-translational
      modifications

      Phosphorylated; activates the channel. It is not clear whether PKC phosphorylation itself activates the channel or permits activation by phosphorylation at PKA sites.
      Ubiquitinated, leading to its degradation in the lysosome. Deubiquitination by USP10 in early endosomes, enhances its endocytic recycling.
    • Cellular localization

      Early endosome membrane.
    • Target information above from: UniProt accession P13569 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 463674 Chimpanzee
      • Entrez Gene: 281067 Cow
      • Entrez Gene: 102130331 Cynomolgus monkey
      • Entrez Gene: 492302 Dog
      • Entrez Gene: 101132066 Gorilla
      • Entrez Gene: 1080 Human
      • Entrez Gene: 403154 Pig
      • Entrez Gene: 100009471 Rabbit
      • Entrez Gene: 24255 Rat
      • Entrez Gene: 574346 Rhesus monkey
      • Entrez Gene: 443347 Sheep
      • Omim: 602421 Human
      • SwissProt: Q2QLE5 Chimpanzee
      • SwissProt: P35071 Cow
      • SwissProt: Q7JII8 Cynomolgus monkey
      • SwissProt: Q5U820 Dog
      • SwissProt: Q2IBF6 Gorilla
      • SwissProt: P13569 Human
      • SwissProt: Q6PQZ2 Pig
      • SwissProt: Q00554 Rabbit
      • SwissProt: P34158 Rat
      • SwissProt: Q00553 Rhesus monkey
      • SwissProt: Q00555 Sheep
      • Unigene: 489786 Human
      • Unigene: 621460 Human
      • Unigene: 661104 Human
      • Unigene: 124539 Rat
      see all
    • Alternative names

      • ABC 35 antibody
      • ABC35 antibody
      • ABCC 7 antibody
      • ABCC7 antibody
      • ATP binding cassette sub family C member 7 antibody
      • ATP Binding Cassette Superfamily C Member 7 antibody
      • ATP binding cassette transporter sub family C member 7 antibody
      • ATP-binding cassette sub-family C member 7 antibody
      • cAMP dependent chloride channel antibody
      • cAMP-dependent chloride channel antibody
      • CF antibody
      • CFTR antibody
      • CFTR/MRP antibody
      • CFTR_HUMAN antibody
      • Channel conductance controlling ATPase antibody
      • Channel conductance-controlling ATPase antibody
      • Cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub family C, member 7) antibody
      • Cystic fibrosis transmembrane conductance regulator antibody
      • Cystic fibrosis transmembrane conductance regulator ATP binding cassette sub family C member 7 antibody
      • Cystic Fibrosis Transmembrane Regulator antibody
      • dJ760C5.1 antibody
      • MRP 7 antibody
      • MRP7 antibody
      • TNR CFTR antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-CFTR antibody (ab2916)
      Immunocytochemistry/ Immunofluorescence - Anti-CFTR antibody (ab2916)

      Immunolocalization of CFTR in HEK293 cells stably transfected with the CFTR gene using ab2916.

    Protocols

    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (3)

    Publishing research using ab2916? Please let us know so that we can cite the reference in this datasheet.

    ab2916 has been referenced in 3 publications.

    • Li W  et al. CFTR inhibits the invasion and growth of esophageal cancer cells by inhibiting the expression of NF-?B. Cell Biol Int 42:1680-1687 (2018). PubMed: 30358020
    • Yang X  et al. High CFTR expression in Philadelphia chromosome-positive acute leukemia protects and maintains continuous activation of BCR-ABL and related signaling pathways in combination with PP2A. Oncotarget 8:24437-24448 (2017). WB . PubMed: 28445932
    • Klein T  et al. Investigation and characterization of the duct cell-enriching process during serum-free suspension and monolayer culture using the human exocrine pancreas fraction. Pancreas 38:36-48 (2009). PubMed: 18665014

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-5 of 5 Abreviews or Q&A

    Flow Cytometry abreview for Anti-CFTR antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Human Cell (Lung)
    Permeabilization
    No
    Gating Strategy
    Live, singlets, debris exclusion
    Specification
    Lung
    Fixation
    Paraformaldehyde
    Read More

    Dr. James Xiao

    Verified customer

    Submitted Dec 14 2020

    Question

    A customer just purchased #ab2916 and did immunofluorescence. Unfortunately, he cannot get staining fluorescence. Below is the questionnaire. Thank you. 1. Order details: * Batch number: ab2916 * Antibody storage conditions (temperature/reconstitution etc) -20? 2. Please describe the problem (high background, no staining etc). no staining 3. On what material are you testing the antibody in IHC * Species: human * Cell Iine or tissue: sperm 4. How did you fix the samples? * Ethanol, methanol: (was it ice cold) * Acetone: (was it ice cold) * Paraformaldehyde (percentage, perfusion fixed on not): 4%, not perfusion fixed * Fixation time: 40min * Fixation temperature: room temperature 6. Blocking steps: * For HRP detection method: did you block endogenous peroxidases: * How did you block the unspecific binding sites: BSA * For how long: 1h 7. Primary antibody * Specification (in which species was it raised against): rabbit * At what dilution(s) have you tested this antibody: 1:500 * What dilution buffer was used: BSA * Incubation time: 16h * Incubation temperature: 4? * What washing steps were done: PBS 5min, 4times 8. Secondary antibody * Specification (in which species was it raised against): goat * What dilution buffer was used: BSA * Incubation time: 1h * Incubation temperature: room temperature * What washing steps were done: PBS 5min, 4times * Do you know whether the problems you are experiencing come from the secondary? No, not the problem from 2nd antibody 9. What detection method are you using? immunofluorescence 10. Background staining See attachment 11. Which detection system did you use? FITC 12. Did you apply positive and negative controls along with the samples? We applied negative controls, and it’s reported CFTR expressed by sperm and could be detected by immunofluorescence. 13. Optimization attempts * How many times have you tried the IHC? One time * Do you obtain the same results every time? yes * What steps have you altered?

    Read More

    Abcam community

    Verified customer

    Asked on Jul 10 2006

    Answer

    I'm very sorry to hear one of your customers is experiencing problems with ab2916, indeed CFTR is known to be present in sperm and should be detectable with ab2916. I think the problem comes from the fixation which is damaging the epitopes. I would recommend to fix the cells in methanol for 5 minutes at -20C. I enclose below the protocol used by Walker et al (Walker J et al. Production and characterisation of monoclonal and polyclonal antibodies to different regions of the cystic fibrosis transmembrane conductance regulator (CFTR): detection of immunologically related proteins. J Cell Sci 108 (Pt 6):2433-44 (1995)): Wash cells 3x PBS, then fixe and permeabilize by incubation in methanol at -20°C for 5 min. Incubate coverslips in PBS/0.2% BSA for 5 min followed by a PBS wash and incubation with primary antibody in PBS/0.2% BSA for 1 h at room temperature. After washing in PBS/0.2 % BSA to remove excess primary antibody, incubate cells in secondary antibody. Please let me know if the customer still has problems with this protocol and I can offer you a replacement vial or credit note,

    Read More

    Abcam Scientific Support

    Answered on Jul 11 2006

    Question

    DESCRIPTION OF THE PROBLEM I have just tried ab2916 on sheep kidney proteins and got a large number of non-specific bands and no band at the expected 170kDa for CFTR. In your product sheet, Western Blotting was not mentioned, but I noticed that ab2784 is quite the same antibody, and the antigen peptide used to create it is the same as that of ab2916. However, in the ab2784 product sheet, Western Blotting was mentioned. Are there any differences between these two antibodies in terms of whether it would work for immunohistochemistry and/or Western Blotting ?

    Read More

    Abcam community

    Verified customer

    Asked on Jun 23 2005

    Answer

    Thank you for contacting us for some advice on ab2916 and ab2784. You are correct, both antibodies were made using the same immunizing peptide, however they are raised in different species and more importantly we do not know if they recognise the same epitope within aa103-117, so a different epitope could explain the difference. We have not tested ab2916 in WB and from your feedback it does appear that it does not work in that application, would you please consider leaving a review on this antibody so we can add this information for future users? We reward reviews (negative and positive) with 100 Abcam points which can be used for a number of gifts. I hope the above information will help you, please do not hesitate to contact me again if you require further assistance,

    Read More

    Abcam Scientific Support

    Answered on Jun 24 2005

    Question

    can you please tell me if the peptide used to make this antibody was made from the intracellular or extracellular portion of the protein?

    Read More

    Abcam community

    Verified customer

    Asked on Jun 20 2005

    Answer

    Both ab2916 and ab2784 are made from the same synthetic peptide corresponding to amino acid residues 103-117. Ab2784 corresponds to amino acid residues 103-117 found in the first extracellular loop of human and rabbit CFTR. So, it appears that ab2916 was made from the extracellular portion. I hope this information helps; if you have any additional questions, please contact us again.

    Read More

    Abcam Scientific Support

    Answered on Jun 20 2005

    Question

    BATCH NUMBER -- NOT SPECIFIED -- ORDER NUMBER 75819 DESCRIPTION OF THE PROBLEM Multiple bands and no signal at expected size. SAMPLE Sheep kidney and mouse lung PRIMARY ANTIBODY 1:1000 as recommended SECONDARY ANTIBODY anti-rabbit HRP DETECTION METHOD ECL POSITIVE AND NEGATIVE CONTROLS USED Would using human protein as positive control be OK ? What would you recommend as a positive control ? ANTIBODY STORAGE CONDITIONS -20C SAMPLE PREPARATION Tris/EDTA/Triton-X100 AMOUNT OF PROTEIN LOADED 30 micrograms ELECTROPHORESIS/GEL CONDITIONS SDS-PAGE TRANSFER AND BLOCKING CONDITIONS 1hr non-fat milk powder in TTBS/TBS HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 2 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes ADDITIONAL NOTES Which extraction protocol would you recommend so as to extract CFTR proteins that are detectable by the above antibody ?

    Read More

    Abcam community

    Verified customer

    Asked on Apr 15 2005

    Answer

    I'm sorry to hear you are experiencing problems with ab2916. This antibody has not been tested for western blotting, it is therefore difficult to find out what the problem might be. A litterature search about CFTR enabled me to find that the lysis buffer used to extract the protein from K562 cells is the following NP40 based buffer: 50 mM HEPES, 250 mM NaCl, 1 mM EDTA, 1% NP-40, 1 mM PMSF, and 1 mM DTT. This information was from the reference "CFTR in K562 human leukemic cells. Assef YA, Damiano AE, Zotta E, Ibarra C, Kotsias BA. Am J Physiol Cell Physiol. 2003 Aug;285(2):C480-8." The article continues: "Nuclei and unbroken cells were removed with centrifugation (6,000 g for 15 min at 4°C). Total protein in each sample was quantified using the bicinchoninic acid assay (BCA Protein, Pierce Chemical). For Western blot studies, 100 ug of total proteins from K562 cells and 50 ug of total proteins from T84 cells were dissolved in loading buffer (4% sodium dodecil sulfate, 0.125 M Tris-HCl, pH 6.8, 0.2 M dithiothreitol, 0.02% bromophenol blue, and 20% glycerol). The preparation was heated to 90°C for 2 min, resolved on 7.5% polyacrylamide gel, and electrotransferred onto nitrocellulose membranes(Hybond ECL, Amersham Pharmacia Biotech, UK)." I would recommend trying a range of dilutions (e.g. 1/100-1/1000) and incubating the antibody overnight at 4C. I hope the above information will help you, please let me know if you require further assistance,

    Read More

    Abcam Scientific Support

    Answered on Apr 15 2005

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