Anti-CFTR antibody (ab2916)
Key features and details
- Rabbit polyclonal to CFTR
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CFTR antibody
See all CFTR primary antibodies -
Description
Rabbit polyclonal to CFTR -
Host species
Rabbit -
Specificity
Detects cystic fibrosis transmembrane conductance factor (CFTR) from cells overexpressing the human protein. -
Tested Applications & Species
Application Species ICC/IF Human -
Immunogen
Synthetic peptide corresponding to Human CFTR aa 103-117.
Sequence:GRIIASYDPDNKEER
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Primary antibody notes
Cystic Fibrosis (CF) is a common lethal genetic disease caused by mutations of the gene coding for the cystic fibrosis transmembrane conductance factor, a cAMP regulated chloride channel. Approximately 70% of all CF cases share the deletion of a phenylalanine at position 508 (delta F508) which results in abnormal chloride transport. Since the CF mutation is lethal, most often by lung and liver disease, it raises the question of why this genetic disease remains as common as it is. One possible explanation is that Salmonella typhi has been shown to use CFTR to enter intestinal epithelial cells and that delta F508 heterozygote and homozygote mice showed 86% and 100% reductions in S. typhi intestinal submucosal uptake. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab2916 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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ICC/IF |
Human
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All applications |
Rat
Sheep
Rabbit
Guinea pig
Cow
Dog
Pig
Chimpanzee
Monkey
Non human primates
Cynomolgus monkey
Rhesus monkey
Gorilla
Elephant
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Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 1 µg/ml.
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Notes |
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ICC/IF
Use a concentration of 1 µg/ml. |
Target
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Function
Involved in the transport of chloride ions. May regulate bicarbonate secretion and salvage in epithelial cells by regulating the SLC4A7 transporter. -
Tissue specificity
Found on the surface of the epithelial cells that line the lungs and other organs. -
Involvement in disease
Defects in CFTR are the cause of cystic fibrosis (CF) [MIM:219700]; also known as mucoviscidosis. CF is the most common genetic disease in the Caucasian population, with a prevalence of about 1 in 2'000 live births. Inheritance is autosomal recessive. CF is a common generalized disorder of exocrine gland function which impairs clearance of secretions in a variety of organs. It is characterized by the triad of chronic bronchopulmonary disease (with recurrent respiratory infections), pancreatic insufficiency (which leads to malabsorption and growth retardation) and elevated sweat electrolytes.
Defects in CFTR are the cause of congenital bilateral absence of the vas deferens (CBAVD) [MIM:277180]. CBAVD is an important cause of sterility in men and could represent an incomplete form of cystic fibrosis, as the majority of men suffering from cystic fibrosis lack the vas deferens. -
Sequence similarities
Belongs to the ABC transporter superfamily. ABCC family. CFTR transporter (TC 3.A.1.202) subfamily.
Contains 2 ABC transmembrane type-1 domains.
Contains 2 ABC transporter domains. -
Domain
The PDZ-binding motif mediates interactions with GOPC and with the SLC4A7, SLC9A3R1/EBP50 complex. -
Post-translational
modificationsPhosphorylated; activates the channel. It is not clear whether PKC phosphorylation itself activates the channel or permits activation by phosphorylation at PKA sites.
Ubiquitinated, leading to its degradation in the lysosome. Deubiquitination by USP10 in early endosomes, enhances its endocytic recycling. -
Cellular localization
Early endosome membrane. - Information by UniProt
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Database links
- Entrez Gene: 463674 Chimpanzee
- Entrez Gene: 281067 Cow
- Entrez Gene: 102130331 Cynomolgus monkey
- Entrez Gene: 492302 Dog
- Entrez Gene: 101132066 Gorilla
- Entrez Gene: 1080 Human
- Entrez Gene: 403154 Pig
- Entrez Gene: 100009471 Rabbit
see all -
Alternative names
- ABC 35 antibody
- ABC35 antibody
- ABCC 7 antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab2916 has been referenced in 3 publications.
- Li W et al. CFTR inhibits the invasion and growth of esophageal cancer cells by inhibiting the expression of NF-?B. Cell Biol Int 42:1680-1687 (2018). PubMed: 30358020
- Yang X et al. High CFTR expression in Philadelphia chromosome-positive acute leukemia protects and maintains continuous activation of BCR-ABL and related signaling pathways in combination with PP2A. Oncotarget 8:24437-24448 (2017). WB . PubMed: 28445932
- Klein T et al. Investigation and characterization of the duct cell-enriching process during serum-free suspension and monolayer culture using the human exocrine pancreas fraction. Pancreas 38:36-48 (2009). PubMed: 18665014