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Inquiry: Hello, I am setting up an ELISA assay for free-alpha hCG. I have your coating antibody ab11387 and two detection antibodies ab11232 and ab11386. I want to biotinylate the detection antibodies so I can use streptavidin HRP and substrate to produce a signal. Do you have a suitable biotinylation protocol available? I do have in the lab a biotinylation kit from another company. Unfortunately, your detection antibodies are only 1mg/ml and they contain sodium azide. I don't want to have to dialyse the 250µl of antibody. Any ideas? PS: You don't have an inquiry type that covers a technical question.
Asked on Jun 14 2012
Thank you for contacting us with your question.
We do have abiotinylation kit thatis suitable to use with these detection antibodies, ab102864-
For this kit, the minimum starting concentration is 1 mg/mL, and azide up to 0.1% has negligible effect. The kit contains 3 vials that can each conjugate 100 uL of antibody, so you would need a couple of kits in order to conjugate the entire contents of ab11232 and ab11386 (250 uL each).
Would this kit work for you? Please let me know if you have further questions or if there is anything else that we can do for you, and I'll be happy to help.
Answered on Jun 14 2012