Key features and details
- Rabbit polyclonal to cGAS
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-cGAS antibody
See all cGAS primary antibodies
DescriptionRabbit polyclonal to cGAS
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
- WB: Rh30 cells IHC-P: Human testis, placenta, tonsil and small intestine tissues.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab224144 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 59 kDa.|
FunctionNucleotidyltransferase that catalyzes the formation of cyclic GMP-AMP (cGAMP) from ATP and GTP. Catalysis involves both the formation of a 2',5' phosphodiester linkage at the GpA step and the formation of a 3',5' phosphodiester linkage at the ApG step, producing c[G(2',5')pA(3',5')p]. Has antiviral activity by acting as a key cytosolic DNA sensor, the presence of double-stranded DNA (dsDNA) in the cytoplasm being a danger signal that triggers the immune responses. Binds cytosolic DNA directly, leading to activation and synthesis of cGAMP, a second messenger that binds to and activates TMEM173/STING, thereby triggering type-I interferon production.
Tissue specificityExpressed in the monocytic cell line THP1.
Sequence similaritiesBelongs to the mab-21 family.
Cellular localizationCytoplasm > cytosol.
- Information by UniProt
- C6orf150 antibody
- cGAMP synthase antibody
- cGAS antibody
All lanes : ab224144 at 0.4 µg/ml
All lanes : Rh30 cells
Lane 1 : Control siRNA
Lane 2 : Target specific siRNA probe
Predicted band size: 59 kDa
Loading control: Anti-GAPDH
Ab224144 staining cGAS of human paraffin-embedded small intestine tissue by immunohistochemcial analysis shows strong granular cytoplasmic positivity in glandular cells.
Ab224144 staining cGAS of human paraffin-embedded tonsil tissue by immunohistochemcial analysis shows moderate to strong granular cytoplasmic positivity in non-germinal center and germinal center cells.
Ab224144 staining cGAS of human paraffin-embedded testis by immunohistochemcial analysis shows strong cytoplasmic positivity in seminiferous cells.
Ab224144 staining cGAS of human paraffin-embedded placenta tissue by immunohistochemcial analysis shows strong granular cytoplasmic positivity in trophoblastic cells.
ab224144 has been referenced in 1 publication.
- Cindrova-Davies T et al. Evidence of oxidative stress-induced senescence in mature, post-mature and pathological human placentas. Placenta 68:15-22 (2018). PubMed: 30055665