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    cgbp-antibody-ab225699.pdf

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Epigenetics and Nuclear Signaling Chromatin Remodeling Trithorax
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Anti-CGBP antibody (ab225699)

  • Datasheet
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Western blot - Anti-CGBP antibody (ab225699)
  • Immunoprecipitation - Anti-CGBP antibody (ab225699)

Key features and details

  • Rabbit polyclonal to CGBP
  • Suitable for: WB, IP
  • Reacts with: Mouse, Human
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-CGBP antibody
    See all CGBP primary antibodies
  • Description

    Rabbit polyclonal to CGBP
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human CGBP aa 550-600. The exact sequence is proprietary. (NP_055408.2).
    Database link: Q9P0U4

  • Positive control

    • WB: HeLa, HEK-293T and NIH/3T3 whole cell lysates. IP: HeLa whole cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab225699 was affinity purified using an epitope specific to CGBP immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Remodeling
    • Trithorax
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Co-factors

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • NIH/3T3 whole cell lysate (ab7179)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab225699 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IP
Human
WB
Human
All applications
Rat
Rabbit
Horse
Cow
Dog
Pig
Xenopus laevis
Chimpanzee
Rhesus monkey
Xenopus tropicalis
Application Abreviews Notes
WB
1/2000 - 1/10000. Predicted molecular weight: 76 kDa.
IP
Use at 2-10 µg/mg of lysate.
Notes
WB
1/2000 - 1/10000. Predicted molecular weight: 76 kDa.
IP
Use at 2-10 µg/mg of lysate.

Target

  • Function

    Transcriptional activator that exhibits a unique DNA binding specificity for [AC]CpG[AC] unmethylated CpG motifs.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Contains 1 CXXC-type zinc finger.
    Contains 1 PHD-type zinc finger.
  • Domain

    The acidic domain carries the potential to activate transcription.
  • Post-translational
    modifications

    May be regulated by proteolysis.
  • Cellular localization

    Nucleus speckle. Associated with euchromatin. During mitosis, excluded from condensed chromosomes.
  • Target information above from: UniProt accession Q9P0U4 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 455419 Chimpanzee
    • Entrez Gene: 511446 Cow
    • Entrez Gene: 480217 Dog
    • Entrez Gene: 100069452 Horse
    • Entrez Gene: 30827 Human
    • Entrez Gene: 74322 Mouse
    • Entrez Gene: 100522911 Pig
    • Entrez Gene: 103349340 Rabbit
    • Entrez Gene: 291440 Rat
    • Entrez Gene: 700341 Rhesus monkey
    • Entrez Gene: 613066 Xenopus tropicalis
    • Omim: 609150 Human
    • SwissProt: Q5EA28 Cow
    • SwissProt: Q9P0U4 Human
    • SwissProt: Q9CWW7 Mouse
    • Unigene: 180933 Human
    • Unigene: 17537 Mouse
    • Unigene: 86349 Rat
    see all
  • Alternative names

    • 2410002I16Rik antibody
    • 5830420C16Rik antibody
    • AI426635 antibody
    • CFP 1 antibody
    • CFP1 antibody
    • CpG binding protein antibody
    • CpG-binding protein antibody
    • CXXC 1 antibody
    • CXXC finger 1 antibody
    • CXXC finger 1 PHD domain antibody
    • CXXC finger protein 1 antibody
    • CXXC type zinc finger protein 1 antibody
    • CXXC-type zinc finger protein 1 antibody
    • CXXC1 antibody
    • CXXC1_HUMAN antibody
    • DNA binding protein with PHD finger and CXXC domain antibody
    • hCGBP antibody
    • HsT2645 antibody
    • PCCX 1 antibody
    • PCCX1 antibody
    • PHD finger and CXXC domain containing protein 1 antibody
    • PHD finger and CXXC domain-containing protein 1 antibody
    • PHF 18 antibody
    • PHF18 antibody
    • Protein containing CXXC domain 1 antibody
    • SPP 1 antibody
    • SPP1 antibody
    • ZCGPC 1 antibody
    • ZCGPC1 antibody
    • Zinc finger, CpG binding type containing 1 antibody
    see all

Images

  • Western blot - Anti-CGBP antibody (ab225699)
    Western blot - Anti-CGBP antibody (ab225699)
    All lanes : Anti-CGBP antibody (ab225699) at 0.04 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg
    Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
    Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 76 kDa


    Exposure time: 3 minutes
  • Immunoprecipitation - Anti-CGBP antibody (ab225699)
    Immunoprecipitation - Anti-CGBP antibody (ab225699)

    CGBP was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab225699 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab225699 at 0.4 µg/ml.

    Lane 1: ab225699 IP in HeLa whole cell lysate.

    Lane 2: Control IgG IP in HeLa whole cell lysate.

    Detection:  Chemiluminescence with exposure time of 10 seconds.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab225699? Please let us know so that we can cite the reference in this datasheet.

    ab225699 has not yet been referenced specifically in any publications.

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