Anti-CGBP antibody (ab225699)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-CGBP antibody
See all CGBP primary antibodies -
Description
Rabbit polyclonal to CGBP -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Xenopus tropicalis
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Immunogen
Synthetic peptide within Human CGBP aa 550-600. The exact sequence is proprietary. (NP_055408.2).
Database link: Q9P0U4 -
Positive control
- WB: HeLa, HEK-293T and NIH/3T3 whole cell lysates. IP: HeLa whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab225699 was affinity purified using an epitope specific to CGBP immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab225699 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/10000. Predicted molecular weight: 76 kDa. | |
| IP | Use at 2-10 µg/mg of lysate. |
Target
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Function
Transcriptional activator that exhibits a unique DNA binding specificity for [AC]CpG[AC] unmethylated CpG motifs. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Contains 1 CXXC-type zinc finger.
Contains 1 PHD-type zinc finger. -
Domain
The acidic domain carries the potential to activate transcription. -
Post-translational
modificationsMay be regulated by proteolysis. -
Cellular localization
Nucleus speckle. Associated with euchromatin. During mitosis, excluded from condensed chromosomes. - Information by UniProt
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Database links
- Entrez Gene: 455419 Chimpanzee
- Entrez Gene: 511446 Cow
- Entrez Gene: 480217 Dog
- Entrez Gene: 100069452 Horse
- Entrez Gene: 30827 Human
- Entrez Gene: 74322 Mouse
- Entrez Gene: 100522911 Pig
- Entrez Gene: 103349340 Rabbit
see all -
Alternative names
- 2410002I16Rik antibody
- 5830420C16Rik antibody
- AI426635 antibody
see all
Images
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Western blot - Anti-CGBP antibody (ab225699)All lanes : Anti-CGBP antibody (ab225699) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 76 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-CGBP antibody (ab225699)CGBP was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab225699 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab225699 at 0.4 µg/ml.
Lane 1: ab225699 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
Datasheets and documents
References
ab225699 has not yet been referenced specifically in any publications.
