Overview

  • Product name
  • Description
    Rabbit polyclonal to CGBP
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Xenopus tropicalis
  • Immunogen

    Synthetic peptide within Human CGBP aa 550-600. The exact sequence is proprietary. (NP_055408.2).
    Database link: Q9P0U4

  • Positive control
    • WB: HeLa, HEK-293T and NIH/3T3 whole cell lysates. IP: HeLa whole cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab225699 was affinity purified using an epitope specific to CGBP immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab225699 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 76 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function
    Transcriptional activator that exhibits a unique DNA binding specificity for [AC]CpG[AC] unmethylated CpG motifs.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Contains 1 CXXC-type zinc finger.
    Contains 1 PHD-type zinc finger.
  • Domain
    The acidic domain carries the potential to activate transcription.
  • Post-translational
    modifications
    May be regulated by proteolysis.
  • Cellular localization
    Nucleus speckle. Associated with euchromatin. During mitosis, excluded from condensed chromosomes.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2410002I16Rik antibody
    • 5830420C16Rik antibody
    • AI426635 antibody
    • CFP 1 antibody
    • CFP1 antibody
    • CpG binding protein antibody
    • CpG-binding protein antibody
    • CXXC 1 antibody
    • CXXC finger 1 antibody
    • CXXC finger 1 PHD domain antibody
    • CXXC finger protein 1 antibody
    • CXXC type zinc finger protein 1 antibody
    • CXXC-type zinc finger protein 1 antibody
    • CXXC1 antibody
    • CXXC1_HUMAN antibody
    • DNA binding protein with PHD finger and CXXC domain antibody
    • hCGBP antibody
    • HsT2645 antibody
    • PCCX 1 antibody
    • PCCX1 antibody
    • PHD finger and CXXC domain containing protein 1 antibody
    • PHD finger and CXXC domain-containing protein 1 antibody
    • PHF 18 antibody
    • PHF18 antibody
    • Protein containing CXXC domain 1 antibody
    • SPP 1 antibody
    • SPP1 antibody
    • ZCGPC 1 antibody
    • ZCGPC1 antibody
    • Zinc finger, CpG binding type containing 1 antibody
    see all

Images

  • All lanes : Anti-CGBP antibody (ab225699) at 0.04 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg
    Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
    Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 76 kDa


    Exposure time: 3 minutes
  • CGBP was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab225699 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab225699 at 0.4 µg/ml.

    Lane 1: ab225699 IP in HeLa whole cell lysate.

    Lane 2: Control IgG IP in HeLa whole cell lysate.

    Detection:  Chemiluminescence with exposure time of 10 seconds.

References

ab225699 has not yet been referenced specifically in any publications.

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