Product nameAnti-CGBP antibody
See all CGBP primary antibodies
DescriptionRabbit polyclonal to CGBP
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Xenopus tropicalis
Synthetic peptide within Human CGBP aa 550-600. The exact sequence is proprietary. (NP_055408.2).
Database link: Q9P0U4
- WB: HeLa, HEK-293T and NIH/3T3 whole cell lysates. IP: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab225699 was affinity purified using an epitope specific to CGBP immobilized on solid support.
Our Abpromise guarantee covers the use of ab225699 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 76 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionTranscriptional activator that exhibits a unique DNA binding specificity for [AC]CpG[AC] unmethylated CpG motifs.
Sequence similaritiesContains 1 CXXC-type zinc finger.
Contains 1 PHD-type zinc finger.
DomainThe acidic domain carries the potential to activate transcription.
modificationsMay be regulated by proteolysis.
Cellular localizationNucleus speckle. Associated with euchromatin. During mitosis, excluded from condensed chromosomes.
- Information by UniProt
- 2410002I16Rik antibody
- 5830420C16Rik antibody
- AI426635 antibody
All lanes : Anti-CGBP antibody (ab225699) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 76 kDa
Exposure time: 3 minutes
CGBP was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab225699 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab225699 at 0.4 µg/ml.
Lane 1: ab225699 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
ab225699 has not yet been referenced specifically in any publications.