Anti-CGBP antibody (ab225699)
Key features and details
- Rabbit polyclonal to CGBP
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-CGBP antibody
See all CGBP primary antibodies -
Description
Rabbit polyclonal to CGBP -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
-
Immunogen
Synthetic peptide within Human CGBP aa 550-600. The exact sequence is proprietary. (NP_055408.2).
Database link: Q9P0U4 -
Positive control
- WB: HeLa, HEK-293T and NIH/3T3 whole cell lysates. IP: HeLa whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab225699 was affinity purified using an epitope specific to CGBP immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab225699 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| IP |
Human
|
| WB |
Human
|
| All applications |
Rat
Rabbit
Horse
Cow
Dog
Pig
Xenopus laevis
Chimpanzee
Rhesus monkey
Xenopus tropicalis
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 76 kDa.
|
|
| IP |
Use at 2-10 µg/mg of lysate.
|
| Notes |
|---|
|
WB
1/2000 - 1/10000. Predicted molecular weight: 76 kDa. |
|
IP
Use at 2-10 µg/mg of lysate. |
Target
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Function
Transcriptional activator that exhibits a unique DNA binding specificity for [AC]CpG[AC] unmethylated CpG motifs. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Contains 1 CXXC-type zinc finger.
Contains 1 PHD-type zinc finger. -
Domain
The acidic domain carries the potential to activate transcription. -
Post-translational
modificationsMay be regulated by proteolysis. -
Cellular localization
Nucleus speckle. Associated with euchromatin. During mitosis, excluded from condensed chromosomes. - Information by UniProt
-
Database links
- Entrez Gene: 455419 Chimpanzee
- Entrez Gene: 511446 Cow
- Entrez Gene: 480217 Dog
- Entrez Gene: 100069452 Horse
- Entrez Gene: 30827 Human
- Entrez Gene: 74322 Mouse
- Entrez Gene: 100522911 Pig
- Entrez Gene: 103349340 Rabbit
see all -
Alternative names
- 2410002I16Rik antibody
- 5830420C16Rik antibody
- AI426635 antibody
see all
Images
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Western blot - Anti-CGBP antibody (ab225699)All lanes : Anti-CGBP antibody (ab225699) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 76 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-CGBP antibody (ab225699)CGBP was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab225699 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab225699 at 0.4 µg/ml.
Lane 1: ab225699 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab225699 has not yet been referenced specifically in any publications.
