Anti-CGGBP1 antibody (ab176814)
Key features and details
- Rabbit polyclonal to CGGBP1
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-CGGBP1 antibody
See all CGGBP1 primary antibodies -
Description
Rabbit polyclonal to CGGBP1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
-
Immunogen
Synthetic peptide within Human CGGBP1 aa 1-50 (N terminal). The exact sequence is proprietary. NP_001008391.1
Sequence:MERFVVTAPP ARNRSKTALY VTPLDRVTEF GGELHEDGGK LFCTSCNVVL
Database link: Q9UFW8 -
Positive control
- 293T, HeLa, Jurkat, mouse TCMK1, and mouse NIH 3T3 whole cell lysates.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7-8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab176814 was affinity purified using an epitope specific to CGGBP1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab176814 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| IP |
Human
|
| WB |
Human
|
| All applications |
Rabbit
Horse
Cow
Dog
Chimpanzee
Rhesus monkey
Gorilla
Orangutan
|
| Application | Abreviews | Notes |
|---|---|---|
| IP |
Use at 2-10 µg/mg of lysate.
|
|
| WB |
1/1000 - 1/5000. Predicted molecular weight: 19 kDa.
|
| Notes |
|---|
|
IP
Use at 2-10 µg/mg of lysate. |
|
WB
1/1000 - 1/5000. Predicted molecular weight: 19 kDa. |
Target
-
Function
Binds to nonmethylated 5'-d(CGG)(n)-3' trinucleotide repeats in the FMR1 promoter. May play a role in regulating FMR1 promoter. -
Tissue specificity
Ubiquitous. Highly expressed in placenta, thymus, lymph nodes, cerebellum and cerebral cortex. Low expression in other regions of the brain. -
Developmental stage
Expressed in fetal brain and kidney. Lower expression in fetal liver and lung. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 735840 Chimpanzee
- Entrez Gene: 613824 Cow
- Entrez Gene: 478375 Dog
- Entrez Gene: 101142721 Gorilla
- Entrez Gene: 8545 Human
- Entrez Gene: 106143 Mouse
- Entrez Gene: 693920 Rhesus monkey
- Omim: 603363 Human
see all -
Alternative names
- 20 kDa CGG binding protein antibody
- 20 kDa CGG-binding protein antibody
- CGBP1_HUMAN antibody
see all
Images
-
Immunoprecipitation - Anti-CGGBP1 antibody (ab176814)
Detection of CGGBP1 by Western Blot of Immunprecipitate.
ab176814 at 1µg/ml labeling CGGBP1 in 293T whole cell lysate immunoprecipitated using ab176814 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds. -
Western blot - Anti-CGGBP1 antibody (ab176814)All lanes : Anti-CGGBP1 antibody (ab176814) at 0.4 µg/ml
Lane 1 : 293T whole cell lysates
Lane 2 : HeLa whole cell lysates
Lane 3 : Jurkat whole cell lysates
Lane 4 : mouse TCMK1 whole cell lysates
Lane 5 : mouse NIH 3T3 whole cell lysates
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 19 kDa
Exposure time: 10 seconds
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab176814 has not yet been referenced specifically in any publications.