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    cggbp1-antibody-ab176814.pdf

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Epigenetics and Nuclear Signaling Chromatin Binding Proteins DNA / RNA binding
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Anti-CGGBP1 antibody (ab176814)

  • Datasheet
  • SDS
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Immunoprecipitation - Anti-CGGBP1 antibody (ab176814)
  • Western blot - Anti-CGGBP1 antibody (ab176814)

Key features and details

  • Rabbit polyclonal to CGGBP1
  • Suitable for: IP, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-CGGBP1 antibody
    See all CGGBP1 primary antibodies
  • Description

    Rabbit polyclonal to CGGBP1
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human CGGBP1 aa 1-50 (N terminal). The exact sequence is proprietary. NP_001008391.1
    Sequence:

    MERFVVTAPP ARNRSKTALY VTPLDRVTEF GGELHEDGGK LFCTSCNVVL


    Database link: Q9UFW8
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • 293T, HeLa, Jurkat, mouse TCMK1, and mouse NIH 3T3 whole cell lysates.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7-8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab176814 was affinity purified using an epitope specific to CGGBP1 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Binding Proteins
    • DNA / RNA binding

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • Jurkat whole cell lysate (ab30128)
    • NIH/3T3 whole cell lysate (ab7179)
    • Jurkat whole cell lysate (ab7899)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab176814 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IP
Human
WB
Human
All applications
Rabbit
Horse
Cow
Dog
Chimpanzee
Rhesus monkey
Gorilla
Orangutan
Application Abreviews Notes
IP
Use at 2-10 µg/mg of lysate.
WB
1/1000 - 1/5000. Predicted molecular weight: 19 kDa.
Notes
IP
Use at 2-10 µg/mg of lysate.
WB
1/1000 - 1/5000. Predicted molecular weight: 19 kDa.

Target

  • Function

    Binds to nonmethylated 5'-d(CGG)(n)-3' trinucleotide repeats in the FMR1 promoter. May play a role in regulating FMR1 promoter.
  • Tissue specificity

    Ubiquitous. Highly expressed in placenta, thymus, lymph nodes, cerebellum and cerebral cortex. Low expression in other regions of the brain.
  • Developmental stage

    Expressed in fetal brain and kidney. Lower expression in fetal liver and lung.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession Q9UFW8 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 735840 Chimpanzee
    • Entrez Gene: 613824 Cow
    • Entrez Gene: 478375 Dog
    • Entrez Gene: 101142721 Gorilla
    • Entrez Gene: 8545 Human
    • Entrez Gene: 106143 Mouse
    • Entrez Gene: 693920 Rhesus monkey
    • Omim: 603363 Human
    • SwissProt: Q9UFW8 Human
    • SwissProt: Q8BHG9 Mouse
    • Unigene: 444818 Human
    • Unigene: 10160 Mouse
    see all
  • Alternative names

    • 20 kDa CGG binding protein antibody
    • 20 kDa CGG-binding protein antibody
    • CGBP1_HUMAN antibody
    • CGG binding protein 1 antibody
    • CGG triplet repeat binding protein 1 antibody
    • CGG triplet repeat-binding protein 1 antibody
    • CGG-binding protein 1 antibody
    • CGGBP 1 antibody
    • CGGBP antibody
    • Cggbp1 antibody
    • OTTHUMP00000213853 antibody
    • OTTHUMP00000213877 antibody
    • p20 CGG binding protein antibody
    • p20 CGGBP antibody
    • p20 CGGBP DNA binding protein antibody
    • p20-CGGBP DNA-binding protein antibody
    see all

Images

  • Immunoprecipitation - Anti-CGGBP1 antibody (ab176814)
    Immunoprecipitation - Anti-CGGBP1 antibody (ab176814)

    Detection of CGGBP1 by Western Blot of Immunprecipitate.
    ab176814 at 1µg/ml labeling CGGBP1 in 293T whole cell lysate immunoprecipitated using ab176814 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.

  • Western blot - Anti-CGGBP1 antibody (ab176814)
    Western blot - Anti-CGGBP1 antibody (ab176814)
    All lanes : Anti-CGGBP1 antibody (ab176814) at 0.4 µg/ml

    Lane 1 : 293T whole cell lysates
    Lane 2 : HeLa whole cell lysates
    Lane 3 : Jurkat whole cell lysates
    Lane 4 : mouse TCMK1 whole cell lysates
    Lane 5 : mouse NIH 3T3 whole cell lysates

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 19 kDa


    Exposure time: 10 seconds

Protocols

  • Western blot protocols
  • Immunoprecipitation protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab176814? Please let us know so that we can cite the reference in this datasheet.

ab176814 has not yet been referenced specifically in any publications.

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