Anti-CGGBP1 antibody (ab176814)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-CGGBP1 antibody
See all CGGBP1 primary antibodies -
Description
Rabbit polyclonal to CGGBP1 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rabbit, Horse, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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Immunogen
Synthetic peptide within Human CGGBP1 aa 1-50 (N terminal). The exact sequence is proprietary. NP_001008391.1
Sequence:MERFVVTAPP ARNRSKTALY VTPLDRVTEF GGELHEDGGK LFCTSCNVVL
Database link: Q9UFW8 -
Positive control
- 293T, HeLa, Jurkat, mouse TCMK1, and mouse NIH 3T3 whole cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7-8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab176814 was affinity purified using an epitope specific to CGGBP1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab176814 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at 2-10 µg/mg of lysate. | |
| WB | 1/1000 - 1/5000. Predicted molecular weight: 19 kDa. |
Target
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Function
Binds to nonmethylated 5'-d(CGG)(n)-3' trinucleotide repeats in the FMR1 promoter. May play a role in regulating FMR1 promoter. -
Tissue specificity
Ubiquitous. Highly expressed in placenta, thymus, lymph nodes, cerebellum and cerebral cortex. Low expression in other regions of the brain. -
Developmental stage
Expressed in fetal brain and kidney. Lower expression in fetal liver and lung. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 735840 Chimpanzee
- Entrez Gene: 613824 Cow
- Entrez Gene: 478375 Dog
- Entrez Gene: 101142721 Gorilla
- Entrez Gene: 8545 Human
- Entrez Gene: 106143 Mouse
- Entrez Gene: 693920 Rhesus monkey
- Omim: 603363 Human
see all -
Alternative names
- 20 kDa CGG binding protein antibody
- 20 kDa CGG-binding protein antibody
- CGBP1_HUMAN antibody
see all
Images
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Immunoprecipitation - Anti-CGGBP1 antibody (ab176814)Detection of CGGBP1 by Western Blot of Immunprecipitate.
ab176814 at 1µg/ml labeling CGGBP1 in 293T whole cell lysate immunoprecipitated using ab176814 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds. -
Western blot - Anti-CGGBP1 antibody (ab176814)All lanes : Anti-CGGBP1 antibody (ab176814) at 0.4 µg/ml
Lane 1 : 293T whole cell lysates
Lane 2 : HeLa whole cell lysates
Lane 3 : Jurkat whole cell lysates
Lane 4 : mouse TCMK1 whole cell lysates
Lane 5 : mouse NIH 3T3 whole cell lysates
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 19 kDa
Exposure time: 10 seconds
Datasheets and documents
References
ab176814 has not yet been referenced specifically in any publications.
