Overview

  • Product name
    Anti-cGMP antibody
    See all cGMP primary antibodies
  • Description
    Sheep polyclonal to cGMP
  • Host species
    Sheep
  • Specificity
    This antibody reacts specifically with cyclic GMP.
  • Tested applications
    Suitable for: ELISA, RIAmore details
  • Immunogen

    Succinylated cGMP conjugated to bovine serum albumin.

Properties

Applications

Our Abpromise guarantee covers the use of ab836 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
RIA Use at an assay dependent concentration. Able to detect and quantitate cGMP; 50% binding is achieved with a 1:6,400 dilution.
AP Use at an assay dependent concentration.

Target

  • Relevance
    Cyclic guanosine monophosphate (cGMP) serves as a second messenger in a manner similar to that observed with cAMP. Peptide hormones, such as the natriuretic factors, activate receptors that are associated with membrane-bound guanylate cyclase (GC). Receptor activation of GC leads to the conversion of GTP to cGMP. Nitric oxide (NO) also stimulates cGMP production by activating soluble GC, perhaps by binding to the heme moiety of the enzyme. Similar to cAMP, cGMP mediates most of its intracellular effects through the activation of specific cGMP dependent protein kinases (PKG).
  • Alternative names
    • Cyclic GMP antibody
    • Cyclic guanosine monophosphate antibody
    • Guanosine 3 5 Cyclic Monophosphate antibody

References

This product has been referenced in:
  • Yu L  et al. A novel bioassay for the activity determination of therapeutic human brain natriuretic peptide (BNP). PLoS One 7:e49934 (2012). ELISA . Read more (PubMed: 23185490) »

See 1 Publication for this product

Publishing research using ab836? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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3 reviews or Q&A

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I ordered this antibody and should have received 100 ul, but only received 20 ul.

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
I am sorry that you did not receive the total volume purchased. As requested, I have issued a free of c...

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I am planning to purchase your anti-cGMP antibody for cGMP RIA. According to your website, it is not necessary to succinylate cGMP for the immune reaction. Does it mean that your antibody does not bind to succinylated cGMP? My iodine-labeled cGMP has s...

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Thank you for contacting us. Here is the information I received from our source of this antibody: The anti-cyclic GMP antiserum does recognize succinylated cGMP. Unfortunately, I do not have the protocol used to generate these data. However, the smal...

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Hello, When you use this antibody in immunoassays do you have to acetylate the samples to get good sensitivity since the immunogen was succinylated? Elizabeth Kenten

For the development of this sheep antibody to cGMP, cGMP was succinylated in order to create a site to which BSA could be conjugated, thus creating the antigen that was used to immunize sheep. However, no treatment of samples is necessary in order for...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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