Recombinant Anti-cGMP antibody [EPR10355] (ab169753)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10355] to cGMP
- Suitable for: Competitive ELISA
- Reacts with: Species independent
Related conjugates and formulations
Overview
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Product name
Anti-cGMP antibody [EPR10355]
See all cGMP primary antibodies -
Description
Rabbit monoclonal [EPR10355] to cGMP -
Host species
Rabbit -
Tested applications
Suitable for: Competitive ELISAmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P,IP or WB -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule corresponding to cGMP conjugated to keyhole limpet haemocyanin.
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General notes
The product is specific for cGMP and as tested it does not cross-react with cAMP, AMP, ADP, ATP, cGMP, GMP, GDP, GTP and cCMP.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR10355 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab169753 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Competitive ELISA |
Use a concentration of 0.1 - 4 µg/ml.
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Notes |
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Competitive ELISA
Use a concentration of 0.1 - 4 µg/ml. |
Target
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Relevance
Cyclic guanosine monophosphate (cGMP) serves as a second messenger in a manner similar to that observed with cAMP. Peptide hormones, such as the natriuretic factors, activate receptors that are associated with membrane-bound guanylate cyclase (GC). Receptor activation of GC leads to the conversion of GTP to cGMP. Nitric oxide (NO) also stimulates cGMP production by activating soluble GC, perhaps by binding to the heme moiety of the enzyme. Similar to cAMP, cGMP mediates most of its intracellular effects through the activation of specific cGMP dependent protein kinases (PKG). -
Alternative names
- Cyclic GMP antibody
- Cyclic guanosine monophosphate antibody
- Guanosine 3 5 Cyclic Monophosphate antibody
Images
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2 µg/ml of Goat anti-rabbit IgG (50 µl/well) was coated into 96-wells. After washing with 0.1 % Tween-20, 0.1 µg/ml of ab169753 (50 µl/well) was added and incubated for 1 hour. After washing, 0, 8, 48, 64, 96, 128, 256 and 1000 ng/ml of cAMP, AMP, ADP, ATP, cGMP, GMP, GDP, GTP, cIMP and cCMP (25 µl/well) were added along with 25 µl of the 1/50 diluted HRP conjugated cGMP. After washing, TMB was used to develop the color.
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2µg/ml of goat-anti-rabbit IgG (50µl/well) was coated into 96-wells. After washing with 0.1% Tween-20, 0.2µg/ml of ab169753 (50 µl / well) was added and incubated for 1hr. Then 62.5ng/ml of cAMP, AMP, ADP, ATP, cGMP, GMP, GDP, GTP, cIMP, cCMP and H2O (25 µl / well each) were added, respectively. Finally 25 µl of 1/50 diluted HRP conjugated cGMP was added in each well. TMB was used to develop the color.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab169753 has not yet been referenced specifically in any publications.