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Immunohistochemistry (Frozen sections)
Rat Tissue sections (DRG neurons)
Yes - 0.3% TritonX in 0.1% PBS
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Other product details
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.3% TritonX with 0.1x PBS- 10% Donkeys
Non-Abcam antibody was used: anti - mouse Alexa Fluro 568 Host species: Donkey Clonality: Monoclonal Conjugation: Alexa Fluor® 568
IHC/IF image of ab81887 stained adult DRG neurons. The sections were 4% formaldehyde fixed in 0.3% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The sections were then incubated with the antibody (ab81887) overnight at +4°C. The secondary antibody (red) was Alexa Fluor® 568 donkey anti-mouse used at a 1/1000 dilution for 1h. CGRP expressed in the certain types of neurons mainly in the peri-nuclear location.
Dr. Ruma Raha-Chowdhury
Submitted Mar 21 2012
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