Anti-CGRP antibody [4901] (ab81887)
- Datasheet
- References (38)
- Protocols
Overview
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Product nameAnti-CGRP antibody [4901]
See all CGRP primary antibodies -
DescriptionMouse monoclonal [4901] to CGRP
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Host speciesMouse
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Specificityab81887 is specific for Calcitonin Gene Related Peptide. It also recognizes rat beta CGRP and human alpha CGRP (10 fold less sensitivity compared to rat alpha CGRP).
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Tested applicationsSuitable for: Neutralising, ICC/IF, Flow Cyt, IHC-Frmore details
Unsuitable for: WB -
Species reactivityReacts with: Mouse, Rat, Guinea pig, Human
Predicted to work with: Mammals
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Immunogen
Rat alpha-CGRP
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Positive control
- Dorsal root ganglia, pancreas and gut.
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General notes
This antibody is an excellent tool for immunohistochemistry and for multiple labeling immunofluorescence of peripheral tissue like stomach, intestine, pancreas, sensory ganglia, and all the regions of the body that are innervated by primary afferent neurons as well as central nervous tissue.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferpH: 7.50
Constituents: 0.01% BSA, 50% Glycerol, 0.87% Sodium chloride, 0.238% HEPES -
Concentration information loading... -
PurityProtein G purified
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Primary antibody notesThis antibody is an excellent tool for immunohistochemistry and for multiple labeling immunofluorescence of peripheral tissue like stomach, intestine, pancreas, sensory ganglia, and all the regions of the body that are innervated by primary afferent neurons as well as central nervous tissue.
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ClonalityMonoclonal
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Clone number4901
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IsotypeIgG2a
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Research areas
Associated products
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Compatible Secondaries
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Immunohistochemistry kits
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab81887 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Neutralising | Use at an assay dependent concentration. | |
| ICC/IF | 1/50 - 1/100. | |
| Flow Cyt | Use at an assay dependent concentration. PubMed: 24430801 ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
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| IHC-Fr | 1/1000. |
Target
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RelevanceCGRP is present in C-cells of the thyroid and in central and peripheral nerves. CGRP has several important physiologic roles: it is a potent vasodilator, and can affect the force and rate of heart beat; it can modulate acetylcholine receptor function at the neuromuscular junction; it has been demonstrated to block tolerance to morphine; and it can modulate antigen presentation in Langerhans cells in the skin. Despite these important physiologic functions, therapeutic strategies using CGRP have been impeded due to the lack of a cloned CGRP receptor with which ligands could be developed.
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Cellular localizationEndoplasmic reticulum and Secreted
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Database links
- Entrez Gene: 796 Human
- Entrez Gene: 797 Human
- Entrez Gene: 116903 Mouse
- Entrez Gene: 12310 Mouse
- Entrez Gene: 171519 Rat
- Entrez Gene: 24241 Rat
- Omim: 114130 Human
- Omim: 114160 Human
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Alternative names
- Calcitonin/calcitonin related polypeptide alpha antibody
- Alpha type CGRP antibody
- Beta type CGRP antibody
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Images
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![Immunohistochemistry (Frozen sections) - Anti-CGRP antibody [4901] (ab81887)](https://www.abcam.com/ps/products/81/ab81887/Images/CGRP-Primary-antibodies-ab81887-9.jpg)
Immunohistochemistry (Frozen sections) - Anti-CGRP antibody [4901] (ab81887)This image is courtesy of an Abreview submitted by Ruma Raha-Chowdhuryab81887 staining rat DRG sections by IHC-Fr. The sections were fixed with 4% paraformaldehyde, permeabilised in 0.3% PBS-Tween and blocked for non-specific protein-protein interactions with 10% donkey's serum for 1h at 24°C. Staining with ab81887 at a 1/1000 dilution in PBS-Triton (0.3%) with 10% donkey's serum and 0.02% azide was performed for 24h at 4°C. A donkey anti-mouse Alexa568 polyclonal antibody at 1/1000 was used as the secondary antibody.
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![Immunocytochemistry/ Immunofluorescence - Anti-CGRP antibody [4901] (ab81887)](https://www.abcam.com/ps/products/81/ab81887/Images/CGRP-Primary-antibodies-ab81887-3.gif)
Immunocytochemistry/ Immunofluorescence - Anti-CGRP antibody [4901] (ab81887)Immunofluorescent staining of CGRP (red) in cryopreserved DRG neurons grown on coverslips using ab81887 at 1/50 dilution. Green staining is PGP9.5. Yellow staining is the colocalization of CGRP and PGP9.5. -
![Immunohistochemistry (Frozen sections) - Anti-CGRP antibody [4901] (ab81887)](https://www.abcam.com/ps/products/81/ab81887/Images/CGRP-Primary-antibodies-ab81887-6.jpg)
Immunohistochemistry (Frozen sections) - Anti-CGRP antibody [4901] (ab81887)This image is courtesy of an abreview submitted by Dr. Iwasakiab81887 staining CGRP in Mouse dorsal root ganglion tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 2% BSA and 2% goat serum for 30 minutes at room temperature. Samples were incubated with primary antibody (1/300 in blocking solution) for 12 hours at 4°C. An undiluted Alexa Fluor®488-conjugated Goat anti-mouse IgG polyclonal (2 mg/ml) was used as the secondary antibody.
Protocols
References
This product has been referenced in:
- Chen H et al. Prostaglandin E2 mediates sensory nerve regulation of bone homeostasis. Nat Commun 10:181 (2019). Read more (PubMed: 30643142) »
- Chen JY et al. Parabrachial CGRP Neurons Establish and Sustain Aversive Taste Memories. Neuron 100:891-899.e5 (2018). Read more (PubMed: 30344042) »
