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    cgrp-antibody-4901-ab81887.pdf

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Anti-CGRP antibody [4901] (ab81887)

  • Datasheet
  • SDS
Reviews (9)Q&A (1)References (97)

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Immunocytochemistry/ Immunofluorescence - Anti-CGRP antibody [4901] (ab81887)

    Key features and details

    • Mouse monoclonal [4901] to CGRP
    • Suitable for: ICC/IF, IHC-Fr, Flow Cyt
    • Reacts with: Mouse, Rat, Guinea pig, Human
    • Isotype: IgG2a

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    Overview

    • Product name

      Anti-CGRP antibody [4901]
      See all CGRP primary antibodies
    • Description

      Mouse monoclonal [4901] to CGRP
    • Host species

      Mouse
    • Specificity

      ab81887 is specific for Calcitonin Gene Related Peptide. It also recognizes rat beta CGRP and human alpha CGRP (10 fold less sensitivity compared to rat alpha CGRP).
    • Tested applications

      Suitable for: ICC/IF, IHC-Fr, Flow Cytmore details
      Unsuitable for: WB
    • Species reactivity

      Reacts with: Mouse, Rat, Guinea pig, Human
      Predicted to work with: Mammals
    • Immunogen

      Full length protein corresponding to Rat CGRP aa 1 to the C-terminus.
      Database link: P01256

    • Positive control

      • Dorsal root ganglia
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
    • Storage buffer

      pH: 7.50
      Constituents: 0.01% BSA, 50% Glycerol, 0.87% Sodium chloride, 0.238% HEPES
    • Concentration information loading...
    • Purity

      Protein G purified
    • Primary antibody notes

      This antibody is an excellent tool for immunohistochemistry and for multiple labeling immunofluorescence of peripheral tissue like stomach, intestine, pancreas, sensory ganglia, and all the regions of the body that are innervated by primary afferent neurons as well as central nervous tissue.
    • Clonality

      Monoclonal
    • Clone number

      4901
    • Isotype

      IgG2a
    • Research areas

      • Neuroscience
      • Neurotransmitter
      • Neuropeptides
      • Hormones
      • Cardiovascular
      • Heart
      • Contractility
      • Inotropics
      • Cardiovascular
      • Vasculature
      • Vasodilation
      • Other
      • Neuroscience
      • Processes

    Associated products

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)
    • Recombinant Protein

      • Recombinant Human CGRP protein (Tagged) (ab240778)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab81887 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC/IF (1)
    1/50 - 1/100.
    IHC-Fr (4)
    Use at an assay dependent concentration.
    Flow Cyt
    Use at an assay dependent concentration. PubMed: 24430801

    ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

    Notes
    ICC/IF
    1/50 - 1/100.
    IHC-Fr
    Use at an assay dependent concentration.
    Flow Cyt
    Use at an assay dependent concentration. PubMed: 24430801

    ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

    Application notes
    Is unsuitable for WB.

    Target

    • Relevance

      CGRP is present in C-cells of the thyroid and in central and peripheral nerves. CGRP has several important physiologic roles: it is a potent vasodilator, and can affect the force and rate of heart beat; it can modulate acetylcholine receptor function at the neuromuscular junction; it has been demonstrated to block tolerance to morphine; and it can modulate antigen presentation in Langerhans cells in the skin.
    • Cellular localization

      Endoplasmic reticulum and Secreted
    • Database links

      • Entrez Gene: 796 Human
      • Entrez Gene: 797 Human
      • Entrez Gene: 116903 Mouse
      • Entrez Gene: 12310 Mouse
      • Entrez Gene: 171519 Rat
      • Entrez Gene: 24241 Rat
      • Omim: 114130 Human
      • Omim: 114160 Human
      • SwissProt: P06881 Human
      • SwissProt: P10092 Human
      • SwissProt: P70160 Mouse
      • SwissProt: Q99JA0 Mouse
      • SwissProt: P01256 Rat
      • SwissProt: P01257 Rat
      see all
    • Alternative names

      • Calcitonin/calcitonin related polypeptide alpha antibody
      • Alpha type CGRP antibody
      • Beta type CGRP antibody
      • CALC1 antibody
      • CALC2 antibody
      • CALCA antibody
      • CALCB antibody
      • Calcitonin 1 antibody
      • Calcitonin 2 antibody
      • Calcitonin gene related peptide I antibody
      • Calcitonin gene related peptide II antibody
      • Calcitonin related polypeptide alpha antibody
      • Calcitonin related polypeptide beta antibody
      • CGRP antibody
      • CGRP I antibody
      • CGRP II antibody
      • CGRP1 antibody
      • CGRP2 antibody
      • CT antibody
      • Katacalcin antibody
      • KC antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-CGRP antibody [4901] (ab81887)
      Immunocytochemistry/ Immunofluorescence - Anti-CGRP antibody [4901] (ab81887)
      Immunofluorescent staining of CGRP (red) in cryopreserved DRG neurons grown on coverslips using ab81887 at 1/50 dilution. Green staining is PGP9.5. Yellow staining is the colocalization of CGRP and PGP9.5.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (97)

    Publishing research using ab81887? Please let us know so that we can cite the reference in this datasheet.

    ab81887 has been referenced in 97 publications.

    • Shenton FC  et al. Distribution and morphology of sensory and autonomic fibres in the subendocardial plexus of the rat heart. J Anat 238:36-52 (2021). PubMed: 32783212
    • Xie MX  et al. ATF4 selectively regulates heat nociception and contributes to kinesin-mediated TRPM3 trafficking. Nat Commun 12:1401 (2021). PubMed: 33658516
    • Yang HH  et al. Smaller bladder capacity and stronger bladder contractility in patients with ketamine cystitis are associated with elevated TRPV1 and TRPV4. Sci Rep 11:5200 (2021). PubMed: 33664402
    • Sun Q  et al. Parathyroid hormone attenuates osteoarthritis pain by remodeling subchondral bone in mice. Elife 10:N/A (2021). PubMed: 33646122
    • Zhang Y  et al. Therapeutic effects of peripherally administrated neural crest stem cells on pain and spinal cord changes after sciatic nerve transection. Stem Cell Res Ther 12:180 (2021). PubMed: 33722287
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review

    Filter by Application

    Filter by Species

    Filter by Ratings

    1-9 of 9 Abreviews

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CGRP antibody [4901]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Lung)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate buffer pH 6.0. 20 min
    Permeabilization
    Yes - 1X PBS/0.2% Triton X-100
    Specification
    Lung
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jul 09 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CGRP antibody [4901]

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Pig Tissue sections (Liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: buffer ph6, ph9
    Permeabilization
    No
    Specification
    Liver
    Blocking step
    H2O2 as blocking agent for 5 minute(s) · Concentration: 3% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Mrs. Gabi Onken

    Verified customer

    Submitted Dec 31 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CGRP antibody [4901]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Spinal cord)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Specification
    Spinal cord
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Carl Hobbs

    Verified customer

    Submitted May 30 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CGRP antibody [4901]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Spinal cord)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Specification
    Spinal cord
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Carl Hobbs

    Verified customer

    Submitted May 30 2018

    Immunohistochemistry (Frozen sections) abreview for Anti-CGRP antibody [4901]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Nervus Suralis peripheral neurons)
    Permeabilization
    Yes - Triton X-100
    Specification
    Nervus Suralis peripheral neurons
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 23°C
    Fixative
    Acetone
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Mr. Thomas Klein

    Verified customer

    Submitted Sep 11 2015

    Immunocytochemistry/ Immunofluorescence abreview for Anti-CGRP antibody [4901]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Pig Cell (Dorsal root ganglion neuron)
    Permeabilization
    Yes - Triton X
    Specification
    Dorsal root ganglion neuron
    Blocking step
    BSA as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 37°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted May 05 2015

    Immunohistochemistry (Frozen sections) abreview for Anti-CGRP antibody [4901]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
    Sample
    Rat Tissue sections (Dorsal root ganglian)
    Specification
    Dorsal root ganglian
    Permeabilization
    Yes - 0.3% TritonX-100
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Mar 16 2015

    Immunohistochemistry (Frozen sections) abreview for Anti-CGRP antibody [4901]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rat Tissue sections (DRG neurons)
    Specification
    DRG neurons
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.3% TritonX in 0.1% PBS
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Ruma Raha-Chowdhury

    Verified customer

    Submitted Mar 21 2012

    Immunohistochemistry (Frozen sections) abreview for Anti-CGRP antibody [4901]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (dorsal root ganglion)
    Specification
    dorsal root ganglion
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Blocking step
    2% BSA and 2% goat serum as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    有作 岩崎

    Verified customer

    Submitted Feb 04 2011

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