Anti-CGRP antibody [4901] (ab81887)
![Immunocytochemistry/ Immunofluorescence - Anti-CGRP antibody [4901] (ab81887)](https://www.abcam.com/ps/products/81/ab81887/Images/ab81887-96924-anti-cgrp-antibody-4901-immunofluorescence.gif "Immunocytochemistry/ Immunofluorescence - Anti-CGRP antibody [4901] (ab81887)")
Key features and details
- Mouse monoclonal [4901] to CGRP
- Suitable for: ICC/IF, IHC-Fr, Flow Cyt
- Reacts with: Mouse, Rat, Guinea pig, Human
- Isotype: IgG2a
Overview
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Product name
Anti-CGRP antibody [4901]
See all CGRP primary antibodies -
Description
Mouse monoclonal [4901] to CGRP -
Host species
Mouse -
Specificity
ab81887 is specific for Calcitonin Gene Related Peptide. It also recognizes rat beta CGRP and human alpha CGRP (10 fold less sensitivity compared to rat alpha CGRP). -
Tested applications
Suitable for: ICC/IF, IHC-Fr, Flow Cytmore details
Unsuitable for: WB -
Species reactivity
Reacts with: Mouse, Rat, Guinea pig, Human
Predicted to work with: Mammals
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Immunogen
Full length protein corresponding to Rat CGRP aa 1 to the C-terminus.
Database link: P01256 -
Positive control
- Dorsal root ganglia
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.50
Constituents: 0.01% BSA, 50% Glycerol, 0.87% Sodium chloride, 0.238% HEPES -
Concentration information loading... -
Purity
Protein G purified -
Primary antibody notes
This antibody is an excellent tool for immunohistochemistry and for multiple labeling immunofluorescence of peripheral tissue like stomach, intestine, pancreas, sensory ganglia, and all the regions of the body that are innervated by primary afferent neurons as well as central nervous tissue. -
Clonality
Monoclonal -
Clone number
4901 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab81887 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | (1) |
1/50 - 1/100.
|
| IHC-Fr | (4) |
Use at an assay dependent concentration.
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| Flow Cyt |
Use at an assay dependent concentration. PubMed: 24430801
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
ICC/IF
1/50 - 1/100. |
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IHC-Fr
Use at an assay dependent concentration. |
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Flow Cyt
Use at an assay dependent concentration. PubMed: 24430801 ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Target
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Relevance
CGRP is present in C-cells of the thyroid and in central and peripheral nerves. CGRP has several important physiologic roles: it is a potent vasodilator, and can affect the force and rate of heart beat; it can modulate acetylcholine receptor function at the neuromuscular junction; it has been demonstrated to block tolerance to morphine; and it can modulate antigen presentation in Langerhans cells in the skin. -
Cellular localization
Endoplasmic reticulum and Secreted -
Database links
- Entrez Gene: 796 Human
- Entrez Gene: 797 Human
- Entrez Gene: 116903 Mouse
- Entrez Gene: 12310 Mouse
- Entrez Gene: 171519 Rat
- Entrez Gene: 24241 Rat
- Omim: 114130 Human
- Omim: 114160 Human
see all -
Alternative names
- Calcitonin/calcitonin related polypeptide alpha antibody
- Alpha type CGRP antibody
- Beta type CGRP antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-CGRP antibody [4901] (ab81887)Immunofluorescent staining of CGRP (red) in cryopreserved DRG neurons grown on coverslips using ab81887 at 1/50 dilution. Green staining is PGP9.5. Yellow staining is the colocalization of CGRP and PGP9.5.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (97)
ab81887 has been referenced in 97 publications.
- Shenton FC et al. Distribution and morphology of sensory and autonomic fibres in the subendocardial plexus of the rat heart. J Anat 238:36-52 (2021). PubMed: 32783212
- Xie MX et al. ATF4 selectively regulates heat nociception and contributes to kinesin-mediated TRPM3 trafficking. Nat Commun 12:1401 (2021). PubMed: 33658516
- Yang HH et al. Smaller bladder capacity and stronger bladder contractility in patients with ketamine cystitis are associated with elevated TRPV1 and TRPV4. Sci Rep 11:5200 (2021). PubMed: 33664402
- Sun Q et al. Parathyroid hormone attenuates osteoarthritis pain by remodeling subchondral bone in mice. Elife 10:N/A (2021). PubMed: 33646122
- Zhang Y et al. Therapeutic effects of peripherally administrated neural crest stem cells on pain and spinal cord changes after sciatic nerve transection. Stem Cell Res Ther 12:180 (2021). PubMed: 33722287