Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CGRP antibody [4901]

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Spinal cord)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Spinal cord
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: TBS/BSA/azide
Dilution
1/2000

Secondary antibody

Dilution
1/300
Name
Non-Abcam antibody was used: anti mouse IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin

Additional data

Additional Notes
Submitted image shows a typical distribution pattern of expression in the dorsal horn of the spinal cord. Under these conditions, the antibody should be further diluted.

Mr. Carl Hobbs

Verified customer

Submitted May 30 2018

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