Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CGRP antibody [4901]

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Spinal cord)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Spinal cord
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: TBS/BSA/azide
Dilution
1/2000

Secondary antibody

Dilution
1/300
Name
Non-Abcam antibody was used: anti mouse IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin

Additional data

Additional Notes
Image shows a typical distribution pattern of CGRP in the dorsal horn there is background/non-specific staining which should disappear on further dilution.

Mr. Carl Hobbs

Verified customer

Submitted May 30 2018

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