Overview

  • Product name
  • Description
    Goat polyclonal to CGRP
  • Host species
    Goat
  • Specificity
    Antibody is thought to react with whole molecule (1-37) and 23-37 fragment (C term). Specifically visualises CGRP in brain cells.
  • Tested applications
    Suitable for: IHC-FoFr, ELISA, IHC-P, IHC-Frmore details
    Unsuitable for: IHC-R
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Rat CGRP aa 23-37 (C terminal). Conjugated to gamma globulin.
    Sequence:

    VKDNFVPTNVGSEAF

Properties

Applications

Our Abpromise guarantee covers the use of ab36001 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent dilution. PubMed: 19805319
ELISA 1/500 - 1/2500.
IHC-P 1/3000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr 1/200.
  • Application notes
    Is unsuitable for IHC-R.
  • Target

    • Relevance
      CGRP is present in C-cells of the thyroid and in central and peripheral nerves. CGRP has several important physiologic roles: it is a potent vasodilator, and can affect the force and rate of heart beat; it can modulate acetylcholine receptor function at the neuromuscular junction; it has been demonstrated to block tolerance to morphine; and it can modulate antigen presentation in Langerhans cells in the skin. Despite these important physiologic functions, therapeutic strategies using CGRP have been impeded due to the lack of a cloned CGRP receptor with which ligands could be developed.
    • Cellular localization
      Endoplasmic reticulum and Secreted
    • Database links
    • Alternative names
      • Calcitonin/calcitonin related polypeptide alpha antibody
      • Alpha type CGRP antibody
      • Beta type CGRP antibody
      • CALC1 antibody
      • CALC2 antibody
      • CALCA antibody
      • CALCB antibody
      • Calcitonin 1 antibody
      • Calcitonin 2 antibody
      • Calcitonin gene related peptide I antibody
      • Calcitonin gene related peptide II antibody
      • Calcitonin related polypeptide alpha antibody
      • Calcitonin related polypeptide beta antibody
      • CGRP antibody
      • CGRP I antibody
      • CGRP II antibody
      • CGRP1 antibody
      • CGRP2 antibody
      • CT antibody
      • Katacalcin antibody
      • KC antibody
      see all

    Images

    • Expression of CGRP and P2X3 in rat dorsal root ganglia.

      Immunohistochemical detection of CGRP (left panel, ab36001) and P2X3 (middle panel) on cryosections of a dorsal root ganglion. The right panel shows the merged picture; the white arrows indicate neuronal co-expression of CGRP and P2X3.

      Animals were transcardially perfused with 4% paraformaldehyde in phosphate buffer (pH 7.4). The DRGs (Th13-L2 to L6-S1) were dissected out and post-fixed for 30 min. After PBS washing, DRGs were incubated in 0.01M PBS with 20% sucrose at 4°C overnight and embedded in OCT medium for cryopreservation. Cryosections (9 μm) were thaw-mounted on poly-L-lysine-coated microscope slides. As a blocking step, cryosections were incubated for 1 h with 0.01M PBS (pH 7.4) containing 0.05% thimerosal (PBS) supplemented with 10% normal horse serum (NHS) and 1% Triton X-100. Goat polyclonal anti-CGRP (calcitonin gene-related peptide; ab36001, 1/2500; Abcam) and rabbit polyclonal anti-P2X3 (1/500) were diluted in PBS supplemented with 10% NHS and applied overnight at room temperature. After rinsing in PBS, tissues were incubated with Cy3-labeled donkey anti-goat (1/4000) and FITC-labeled donkey anti-rabbit (1/300) diluted in 0.01M PBS (pH 7.4) and again rinsed in PBS.

    • Excised mouse bladders were fixed in 4% (w/v) paraformaldehyde dissolved in 100 mM sodium cacodylate (pH 7.4) buffer for 2 h at room temperature. In some cases, tissue was fixed by 100% methanol (4°C) for 5 min. Fixed tissue was cut into small pieces with a razor blade, cryoprotected, frozen, sectioned (5 µm), and incubated with primary antibodies (1/100 –1/500 dilution) for 2 h at room temperature (CGRP labeled red, afferent nerves). After washing away unbound primary antibody, the sections were incubated with a mixture of Alexa® 488-conjugated secondary antibody (diluted 1/100), rhodamine-phalloidin (1/50), and Topro-3 (1/1,000). The sections were washed with PBS, postfixed with 4% (wt/vol) paraformaldehyde, and mounted under coverslips with p-diaminobenzidine-containing mounting medium. All immunofluorescent localization data shown are representative images of staining performed on at least three individual bladders.

    • IHC-P image of CGRP staining on rat spinal cord sections using ab36001 (1/3000).

      The sections were deparaffinized and subjected to heat mediated antigen retrieval. The sections were then blocked using 1% BSA at 21°C for 10 min. Incubated with primary antibody at 21°C for 2 hours.

      See Abreview

    • ab36001 staining mouse colon tissue sections by IHC-Fr.

      Sections were PFA fixed and blocked in 5% serum for 30 minutes at 24°C prior to incubating with ab36001, diluted 1/200 in PBS + 0.2% Triton X-100, for 18 hours at 4°C. The secondary antibody was an Alexa Fluor® 546 conjugated, diluted 1/200.

      See Abreview

    References

    This product has been referenced in:
    • Fetterly TL  et al. a2A-Adrenergic Receptor Activation Decreases Parabrachial Nucleus Excitatory Drive onto BNST CRF Neurons and Reduces Their Activity In Vivo. J Neurosci 39:472-484 (2019). Read more (PubMed: 30478032) »
    • Liang CC  et al. Improvement in bladder dysfunction after bladder transplantation of amniotic fluid stem cells in diabetic rats. Sci Rep 8:2105 (2018). IHC ; Rat . Read more (PubMed: 29391467) »
    See all 44 Publications for this product

    Customer reviews and Q&As

    1-8 of 8 Abreviews or Q&A

    Application
    IHC - Wholemount
    Sample
    Mouse Tissue (trachea)
    Specification
    trachea

    Abcam user community

    Verified customer

    Submitted Dec 11 2018

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Nerve (DRG))
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: pH 9.0 EDTA
    Permeabilization
    No
    Specification
    Nerve (DRG)
    Blocking step
    PB ab64226 as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Formaldehyde

    Abcam user community

    Verified customer

    Submitted Apr 30 2018

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: pH 9.0 EDTA
    Permeabilization
    No
    Specification
    Brain
    Blocking step
    PB ab64226 as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Formaldehyde

    Abcam user community

    Verified customer

    Submitted Apr 30 2018

    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (Dorsal Root Ganglia)
    Antigen retrieval step
    None
    Permeabilization
    Yes - 0.3% Triton X-100
    Specification
    Dorsal Root Ganglia
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Fixative
    Paraformaldehyde

    Dr. Jean-Marc Guedon

    Verified customer

    Submitted Jun 03 2015

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rat Tissue sections (Spinal cord)
    Specification
    Spinal cord
    Fixative
    Formaldehyde
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C

    Mr. Carl Hobbs

    Verified customer

    Submitted Feb 25 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Spinal cord)
    Specification
    Spinal cord
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C

    Mr. Carl Hobbs

    Verified customer

    Submitted Sep 06 2012

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (brain, spinal cord, muscle w/nerve)
    Specification
    brain, spinal cord, muscle w/nerve
    Fixative
    formalin, 10% neutral buffered
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate, pH6, microwave 2x, 5 min on high
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C

    Abcam user community

    Verified customer

    Submitted Mar 18 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Colon)
    Specification
    Colon
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C

    Abcam user community

    Verified customer

    Submitted Apr 08 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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