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How was the positive control tissue solubilized?
Asked on Nov 03 2011
Thank you for your patience while I looked into the lysate used for testing this antibody. Here is the lab's reply: "The lysate we use for testing this antibody is actually supplied by a collaborator. I don’t have a lot of information about the lysate but it is supplied to us in RIPA buffer so the customer might like to try an extraction using that if he hasn’t already. We dilute the lysate stock further in RIPA buffer prior to use and also add DTT and loading buffer." I hope this information helps. Please do not hesitate to contact us if you need anything further!
Answered on Nov 03 2011