Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CGRP antibody

Good
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Spinal cord)
Specification
Spinal cord
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C

Other product details

Dilution
1/400
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: TBS/BSA?azide

Secondary antibody

Name
Non-Abcam antibody was used: anti Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/250

Additional data

Additional Notes
An appropriate pattern of of immunostaining that is identical to that given by two othe Abs, including ab36001.
This Ab seems to give the most intense labelling but, it additionally immunolabels rbcs, in my hands.
( not that this should be of concern: one should be able to easily identify an rbc!)
Submitted image of T/S spinal cord area where a dorsal root is entering.

Mr. Carl Hobbs

Verified customer

Submitted Sep 06 2012

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