IHC - Wholemount abreview for Anti-CGRP antibody

Inconclusive
Abreviews
Application
IHC - Wholemount
Sample
Mouse Tissue (tongue)
Specification
tongue

Other product details

Dilution
1/100
Incubation time
48 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: normal goat serum, PBS, triton X-100

Secondary antibody

Name
Non-Abcam antibody was used: Alexa Fluor® 647 F(ab')2 Fragment of Goat Anti-Rab
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Dilution
1/200

Additional data

Additional Notes
The green signals came from WGA488 infused in the blood vessel, and cyan areas indicate the possible CGRP signals.
We also try the anti-CGRP from Sigma, yet the patterns we observed in confocal images are completely different. We may need more tests to verify which one is the signal we are interested in.
Abcam response
Thanks for taking the time to submit this review and for your honest feedback!

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Submitted Sep 25 2012

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