Immunohistochemistry free floating abreview for Anti-CGRP antibody

Inconclusive
Abreviews
Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (Brain tissue)
Specification
Brain tissue

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: PBS + 0.3% Triton X100
Dilution
1/100

Secondary antibody

Dilution
1/500
Name
Non-Abcam antibody was used: Donkey anti Rabbit - Jackson
Host species: Donkey
Clonality: Polyclonal
Conjugation: Biotin

Additional data

Additional Notes
Brain perfused with 4% PFA, then overnight in 4% PFA at 4¯ Celsius, then placed in cryopreserve and sliced into 40 ´m sections via vibratome. Tested with and without heat-mediated antigen retrieval. ABC kit (1:250) and then DAB reaction, with nickel added. Antigen retrieval protocol used: 1 hr in citrate buffer pH 6 at 65¯ Celsius
Abcam response
We would like to thank the reviewer for submitting this Abreview for this product. As the product had not yet been tested in this application on this tissue type we were unsure what the result might be and we appreciate the time and effort that the reviewer has put in to testing this product in this application. The customer has received value off a future purchase for performing these tests. We wish to thank them for participating in our AbTrial program.

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Verified customer

Submitted Apr 07 2017

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