Non-Abcam antibody was used: Donkey anti Rabbit - Jackson Host species: Donkey Clonality: Polyclonal Conjugation: Biotin
Brain perfused with 4% PFA, then overnight in 4% PFA at 4¯ Celsius, then placed in cryopreserve and sliced into 40 ´m sections via vibratome. Tested with and without heat-mediated antigen retrieval. ABC kit (1:250) and then DAB reaction, with nickel added. Antigen retrieval protocol used: 1 hr in citrate buffer pH 6 at 65¯ Celsius
We would like to thank the reviewer for submitting this Abreview for this product. As the product had not yet been tested in this application on this tissue type we were unsure what the result might be and we appreciate the time and effort that the reviewer has put in to testing this product in this application. The customer has received value off a future purchase for performing these tests. We wish to thank them for participating in our AbTrial program.
Abcam user community
Submitted Apr 07 2017
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