Overview

  • Product name

  • Description

    Rabbit polyclonal to ch TOG
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human ch TOG aa 1802-2019.
    Database link: Q14008

  • Positive control

    • WB: HeLa, HepG2 and rat brain lysate. IHC-P: Human kidney and brain tissue. ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab236981 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/5000.
IHC-P 1/200 - 1/500.
ICC/IF 1/50 - 1/200.

Target

  • Relevance

    ch-TOG (also known as colonic and hepatic tumor over-expressed protein) is a 218 kD member of the TOG/XMAP215 family containing heat repeats. This protein is localized in the perinuclear cytoplasm during interphase and at the spindle poles and centrosomes during mitosis. Ch-TOG is believed to function in spindle microtubule organization and has been shown to interact with TACC1, TACC3,and tubulin.
  • Cellular localization

    Localized in the perinuclear cytoplasm during interphase and at the spindle poles and centrosomes during mitosis
  • Database links

  • Alternative names

    • Ch TOG protein antibody
    • chTOG antibody
    • CHTOG protein antibody
    • CKAP 5 antibody
    • CKAP5 antibody
    • CKAP5 protein antibody
    • Colonic and hepatic tumor over expressed antibody
    • Colonic and hepatic tumor over expressed protein antibody
    • Colonic and hepatic tumor overexpressed protein antibody
    • Cytoskeleton associated protein 5 antibody
    • FLJ35359 antibody
    • KIAA0097 antibody
    • TOG antibody
    • TOGp antibody
    see all

Images

  • All lanes : Anti-ch TOG antibody (ab236981) at 1/500 dilution

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 3 : Rat brain tissue lysate

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
  • HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for ch TOG (Green) using ab236981 at a 1/133 dilution in ICC/IF.

    The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the primary antibody overnight at 4°C. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L).

  • Paraffin-embedded human kidney tissue stained for ch TOG with ab236981 at a 1/400 dilution in immunohistochemical analysis.

    After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Paraffin-embedded human brain tissue stained for ch TOG with ab236981 at a 1/400 dilution in immunohistochemical analysis.

    After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

References

ab236981 has not yet been referenced specifically in any publications.

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