Overview

  • Product name

  • Description

    Rabbit polyclonal to CH25H
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within C terminal amino acids 190-239 (VTLLGCHPLT TLTFHVVNIW LSVEDHSGYN FPWSTHRLVP FGWYGGVVHH) of Human CH25H (NP_003947).

  • Positive control

    • Human fetal brain lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab133933 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 32 kDa.

Target

  • Relevance

    Function: Catalyzes the formation of 25-hydroxycholesterol from cholesterol, leading to repress cholesterol biosynthetic enzymes. May play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing. In testis, production of 25-hydroxycholesterol by macrophages may play a role in Leydig cell differentiation. Similarity: Belongs to the sterol desaturase family. PTM: N-glycosylated.
  • Cellular localization

    Endoplasmic reticulum membrane
  • Database links

  • Alternative names

    • C25H antibody
    • Ch25h antibody
    • CH25H_HUMAN antibody
    • Cholesterol 25 hydroxylase antibody
    • Cholesterol 25 monooxygenase antibody
    • Cholesterol 25-hydroxylase antibody
    • Cholesterol 25-monooxygenase antibody
    • EC 1.14.99.38 antibody
    • EC=1.14.99.38 antibody
    • h25OH antibody
    see all

Images

  • Anti-CH25H antibody (ab133933) at 1 µg/ml + Human fetal brain lysate

    Predicted band size: 32 kDa



    5% milk was used for blocking.

References

This product has been referenced in:

  • Wu T  et al. Regulating Innate and Adaptive Immunity for Controlling SIV Infection by 25-Hydroxycholesterol. Front Immunol 9:2686 (2018). Read more (PubMed: 30524435) »
See 1 Publication for this product

Customer reviews and Q&As

Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (murine embryo fibroblasts)
Gel Running Conditions
Non-reduced Denaturing (10%)
Loading amount
30 µg
Specification
murine embryo fibroblasts
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Aug 14 2017

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up