Overview

  • Product name

  • Description

    Rabbit polyclonal to CH25H
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat, Human
  • Immunogen

    Synthetic peptide within Human CH25H aa 58-84 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Sequence:

    LDILCSWVPALRRYKIHPDFSPSAQQL


    Database link: O95992

  • Positive control

    • IHC-P: Mouse kidney tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab214295 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance

    Function: Catalyzes the formation of 25-hydroxycholesterol from cholesterol, leading to repress cholesterol biosynthetic enzymes. May play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing. In testis, production of 25-hydroxycholesterol by macrophages may play a role in Leydig cell differentiation. Similarity: Belongs to the sterol desaturase family. PTM: N-glycosylated.
  • Cellular localization

    Endoplasmic reticulum membrane
  • Database links

  • Alternative names

    • C25H antibody
    • Ch25h antibody
    • CH25H_HUMAN antibody
    • Cholesterol 25 hydroxylase antibody
    • Cholesterol 25 monooxygenase antibody
    • Cholesterol 25-hydroxylase antibody
    • Cholesterol 25-monooxygenase antibody
    • EC 1.14.99.38 antibody
    • EC=1.14.99.38 antibody
    • h25OH antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed paraffin-embedded mouse kidney tissue, blocked with 3% hydrogen peroxide at 37°C for 20 minutes, labeling CH25H using ab214295 at a 1/200 dilution overnight at 4°C, followed by conjugation to the secondary antibody for 90 minutes and DAB staining.

References

This product has been referenced in:

  • Chali F  et al. Lipid markers and related transcripts during excitotoxic neurodegeneration in kainate-treated mice. Eur J Neurosci N/A:N/A (2019). Read more (PubMed: 30767299) »
See 1 Publication for this product

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