Key features and details
- Rabbit polyclonal to CHAC1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat
- Isotype: IgG
Product nameAnti-CHAC1 antibody
See all CHAC1 primary antibodies
DescriptionRabbit polyclonal to CHAC1
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat
Predicted to work with: Human
- Mouse brain lysate; rat brain tissue.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol, 1% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab217808 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100 - 1/1000. Predicted molecular weight: 28 kDa.|
|IHC-P||1/100 - 1/500.|
FunctionCatalyzes the cleavage glutathione into 5-oxoproline and a Cys-Gly dipeptide. Acts specifically on glutathione, but not on other gamma-glutamyl peptides. Glutathione depletion is an important factor for apoptosis initiation and execution (PubMed:23070364). Acts as a pro-apoptotic component of the unfolded protein response pathway by mediating the pro-apoptotic effects of the ATF4-ATF3-DDIT3/CHOP cascade (PubMed:19109178). Negative regulator of Notch signaling pathway involved in embryonic neurogenesis: acts by inhibiting Notch cleavage by furin, maintaining Notch in an immature inactive form, thereby promoting neurogenesis in embryos (PubMed:22445366).
Sequence similaritiesBelongs to the gamma-glutamylcyclotransferase family. ChaC subfamily.
Cellular localizationCytoplasm, cytosol. Golgi apparatus, trans-Golgi network.
- Information by UniProt
- Blocks Notch protein antibody
- Botch antibody
- Cation transport regulator like protein 1 antibody
Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat brain tissue labeling CHAC1 with ab217808 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining
All lanes : Anti-CHAC1 antibody (ab217808) at 1/200 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Mouse kidney lysate
All lanes : Goat Anti-Rabbit IgG Antibody (H+L), HRP at 1/3000 dilution
Predicted band size: 28 kDa
ab217808 has been referenced in 1 publication.
- Allawzi A et al. Redistribution of EC-SOD resolves bleomycin-induced inflammation via increased apoptosis of recruited alveolar macrophages. FASEB J 33:13465-13475 (2019). PubMed: 31560857