Key features and details
- Rabbit polyclonal to CHCH
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CHCH antibody
DescriptionRabbit polyclonal to CHCH
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Cow, Orangutan
- WB: HEK-293T overexpressing CHCH with C-terminal myc-DDk tag (~3.1 kDa). IHC-P: Human colon tissue. ICC/IF: PC-3 cells.
This product was previously labelled as CHURC 1
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab243505 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 13 kDa.|
RelevanceCHURC 1 belongs to the Churchill family. It is a transcriptional activator that mediates FGF signaling during neural development. It plays a role in the regulation of cell movement, but does not bind DNA by itself. There are two named isoforms.
- C14orf52 antibody
- CHCH antibody
- CHURC1 antibody
PFA-fixed, Triton X-100 permeabilized PC-3 (human prostate adenocarcinoma derived from bone) cells stained for CHCH (green) using ab243505 at 4 µg/ml in ICC/IF analysis.
Formalin-fixed, paraffin-embedded human colon tissue stained for CHCH using ab243505 at 1/50 dilution in immunohistochemical analysis.
All lanes : Anti-CHCH antibody (ab243505) at 0.4 µg/ml
Lane 1 : Empty vector transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : HEK-293T overexpressing CHCH with C-terminal myc-DDk tag (~3.1 kDa).
Predicted band size: 13 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab243505 has not yet been referenced specifically in any publications.