Product nameAnti-CHCH antibody
See all CHCH primary antibodies
DescriptionRabbit polyclonal to CHCH
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Cow, Orangutan
- WB: HEK-293T overexpressing CHCH with C-terminal myc-DDk tag (~3.1 kDa). IHC-P: Human colon tissue. ICC/IF: PC-3 cells.
This product was previously labelled as CHURC 1
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab243505 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.4 µg/ml. Predicted molecular weight: 13 kDa.|
RelevanceCHURC 1 belongs to the Churchill family. It is a transcriptional activator that mediates FGF signaling during neural development. It plays a role in the regulation of cell movement, but does not bind DNA by itself. There are two named isoforms.
- C14orf52 antibody
- CHCH antibody
- CHURC1 antibody
PFA-fixed, Triton X-100 permeabilized PC-3 (human prostate adenocarcinoma derived from bone) cells stained for CHCH (green) using ab243505 at 4 µg/ml in ICC/IF analysis.
Formalin-fixed, paraffin-embedded human colon tissue stained for CHCH using ab243505 at 1/50 dilution in immunohistochemical analysis.
All lanes : Anti-CHCH antibody (ab243505) at 0.4 µg/ml
Lane 1 : Empty vector transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : HEK-293T overexpressing CHCH with C-terminal myc-DDk tag (~3.1 kDa).
Predicted band size: 13 kDa
ab243505 has not yet been referenced specifically in any publications.