Key features and details
- Rabbit polyclonal to CHCHD10
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CHCHD10 antibody
See all CHCHD10 primary antibodies
DescriptionRabbit polyclonal to CHCHD10
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
- IHC-P: Human colon, skeletal muscle and liver tissue. ICC/IF: A-431 cells
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab121196 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.|
Sequence similaritiesContains 1 CHCH domain.
- Information by UniProt
- C22orf16 antibody
- CHC10_HUMAN antibody
- CHCHD10 antibody
Immunofluorescent staining of Human cell line A-431 shows positivity in mitochondria. Recommended concentration of ab121196 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemical analysis of human colon tissue sections labeling CHCHD10 with ab121196 at 1/200.
Immunohistochemical analysis of human liver tissue sections labeling CHCHD10 with ab121196 at 1/200.
Immunohistochemical analysis of human skeletal muscle tissue sections labeling CHCHD10 with ab121196 at 1/200.
Immunohistochemical analysis of human skin tissue sections labeling CHCHD10 with ab121196 at 1/200.
ab121196 has been referenced in 2 publications.
- Liu T et al. CHCHD10-regulated OPA1-mitofilin complex mediates TDP-43-induced mitochondrial phenotypes associated with frontotemporal dementia. FASEB J 34:8493-8509 (2020). PubMed: 32369233
- Lehmer C et al. A novel CHCHD10 mutation implicates a Mia40-dependent mitochondrial import deficit in ALS. EMBO Mol Med 10:N/A (2018). WB, ICC/IF . PubMed: 29789341