Overview

  • Product name

    Anti-CHCHD10 antibody
  • Description

    Rabbit polyclonal to CHCHD10
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    antigen sequence, corresponding amino acids 43-142 (GLMAQMATTA AGVAVGSAVG HVMGSALTGA FSGGSSEPSQ PAVQQAPTPA APQPLQMGPC AYEIRQFLDC STTQSDLSLC EGFSEALKQC KYYHGLSSLP) of Human CHCHD10.

  • Positive control

    • IHC-P: Human colon, skeletal muscle and liver tissue. ICC/IF: A-431 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab121196 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Target

  • Sequence similarities

    Contains 1 CHCH domain.
  • Cellular localization

    Mitochondrion.
  • Information by UniProt
  • Database links

  • Alternative names

    • C22orf16 antibody
    • CHC10_HUMAN antibody
    • CHCHD10 antibody
    • Chromosome 22 open reading frame 16 antibody
    • Coiled coil helix coiled coil helix domain containing 10 antibody
    • Coiled coil helix coiled coil helix domain containing protein 10 mitochondrial antibody
    • Coiled-coil-helix-coiled-coil-helix domain-containing protein 10 antibody
    • MGC70831 antibody
    • mitochondrial antibody
    • N27C7-4 antibody
    • OTTHUMP00000198408 antibody
    • OTTHUMP00000198409 antibody
    • Protein N27C7-4 antibody
    see all

Images

  • Immunofluorescent staining of Human cell line A-431 shows positivity in mitochondria. Recommended concentration of ab121196 1-4 µg/ml. Cells treated with PFA/Triton X-100.

  • Immunohistochemical analysis of human colon tissue sections labeling CHCHD10 with ab121196 at 1/200.

     

  • Immunohistochemical analysis of human liver tissue sections labeling CHCHD10 with ab121196 at 1/200.

  • Immunohistochemical analysis of human skeletal muscle tissue sections labeling CHCHD10 with ab121196 at 1/200.

  • Immunohistochemical analysis of human skin tissue sections labeling CHCHD10 with ab121196 at 1/200.

References

This product has been referenced in:

  • Lehmer C  et al. A novel CHCHD10 mutation implicates a Mia40-dependent mitochondrial import deficit in ALS. EMBO Mol Med 10:N/A (2018). WB, ICC/IF . Read more (PubMed: 29789341) »
See 1 Publication for this product

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