Overview

  • Product name
  • Description
    Rabbit polyclonal to CHCHD3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Cow, Pig
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 1 and 214 of Human CHCHD3 (UniProt ID: Q9NX63).

  • Positive control
    • 293T, A431, Jurkat, Raji and mouse liver lysates; Hela xenograft

Properties

Applications

Our Abpromise guarantee covers the use of ab154500 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 26 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Required for maintenance of mitochondrial crista integrity and mitochondrial function. May act as a scaffolding protein that stabilizes protein complexes involved in crista architecture and protein import. Has also been shown to function as a transcription factor which binds to the BAG1 promoter and represses BAG1 transcription.
  • Tissue specificity
    Detected at low levels in brain, placenta, lung, liver, kidney and pancreas with increased levels in heart and skeletal muscle. Higher expression in primary lung cancers than in normal lung tissue.
  • Sequence similarities
    Contains 1 CHCH domain.
  • Cellular localization
    Mitochondrion inner membrane. Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CHCH3_HUMAN antibody
    • CHCHD 3 antibody
    • Chchd3 antibody
    • Coiled coil helix coiled coil helix domain containing 3 antibody
    • Coiled coil helix coiled coil helix domain containing protein 3 mitochondrial antibody
    • Coiled-coil-helix-coiled-coil-helix domain-containing protein 3 antibody
    • FLJ20420 antibody
    • MINOS3 antibody
    • mitochondrial antibody
    • Mitochondrial inner membrane organizing system 3 antibody
    • PPP1R22 antibody
    • Protein phosphatase 1 regulatory subunit 22 antibody
    see all

Images

  • All lanes : Anti-CHCHD3 antibody (ab154500) at 1/5000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 26 kDa



    12% SDS PAGE
  • Anti-CHCHD3 antibody (ab154500) at 1/1000 dilution + mouse liver whole cell lysate at 50 µg

    Predicted band size: 26 kDa



    12% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Hela xenograft, labeling CHCHD3 with ab154500 at 1/500 dilution.

References

ab154500 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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