Overview

  • Product name

  • Description

    Rabbit polyclonal to Chd1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ChIP/Chip, WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chicken
  • Immunogen

    Synthetic peptide within Human Chd1 aa 1660-1710. The exact sequence is proprietary.
    Database link: O14646

  • Positive control

    • WB: HeLa and HEK-293T whole cell lysates. IP: HeLa whole cell lysate. ChIP on ChIP: K562 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab242098 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP/Chip Use at an assay dependent concentration.

10 µg (see Ren et al (Genes Dev. 2002 16: 245-256).

WB 1/2000 - 1/10000.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function

    ATP-dependent chromatin-remodeling factor which functions as substrate recognition component of the transcription regulatory histone acetylation (HAT) complex SAGA. Regulates polymerase II transcription. Also required for efficient transcription by RNA polymerase I, and more specifically the polymerase I transcription termination step. Regulates negatively DNA replication. Not only involved in transcription-related chromatin-remodeling, but also required to maintain a specific chromatin configuration across the genome. Is also associated with histone deacetylase (HDAC) activity (By similarity). Required for the bridging of SNF2, the FACT complex, the PAF complex as well as the U2 snRNP complex to H3K4me3. Functions to modulate the efficiency of pre-mRNA splicing in part through physical bridging of spliceosomal components to H3K4me3. Required for maintaining open chromatin and pluripotency in embryonic stem cells.
  • Sequence similarities

    Belongs to the SNF2/RAD54 helicase family.
    Contains 2 chromo domains.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Domain

    The 2 chromodomains are involved in the binding to the histone H3 methyllysine at position 4 (H3K4me3).
  • Cellular localization

    Nucleus. Cytoplasm. Is released into the cytoplasm when cells enter mitosis and is reincorporated into chromatin during telophase-cytokinesis.
  • Information by UniProt
  • Database links

  • Alternative names

    • ATP dependent helicase CHD 1 antibody
    • ATP-dependent helicase CHD1 antibody
    • CHD 1 antibody
    • CHD-1 antibody
    • Chd1 antibody
    • CHD1_HUMAN antibody
    • Chromodomain helicase DNA binding protein 1 antibody
    • Chromodomain-helicase-DNA-binding protein 1 antibody
    • DKFZp686E2337 antibody
    • OTTHUMP00000222625 antibody
    see all

Images

  • All lanes : Anti-Chd1 antibody (ab242098) at 0.04 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

    Developed using the ECL technique.

    Exposure time: 3 seconds
  • Chd1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab242098 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab242098 at 1 µg/ml.

    Lane 1: ab242098 IP in HeLa whole cell lysate.
    Lane 2: Control IgG IP in HeLa whole cell lysate.

    Detection: Chemiluminescence with exposure time of 10 seconds.

  • ab242098 (10 μg) was used to immunoprecipitate chromatin from K562 cells according to the protocol of Ren et al (Genes Dev. 2002 16: 245-256).

    (A) Immunoprecipitated DNA and reference DNA were amplified via ligation-mediated PCR and the products labeled with fluorescent dUTPs. The labeled ChIP and reference DNA were pooled, hybridized to a DNA microarray, and analyzed. Data points below the +3 SD curve (red line) represent significantly enriched binding sites.

    (B) As a control, a similar experiment was performed using normal rabbit IgG. Compared to the anti-Chd1 ChIP, normal rabbit IgG showed little enrichment.

References

ab242098 has not yet been referenced specifically in any publications.

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