Overview

  • Product name

  • Description

    Rabbit polyclonal to Chd1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Chd1 aa 1500-1550. The exact sequence is proprietary. (NP_001261.2).
    Database link: O14646

  • Positive control

    • IP: HeLa whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab246009 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-5 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function

      ATP-dependent chromatin-remodeling factor which functions as substrate recognition component of the transcription regulatory histone acetylation (HAT) complex SAGA. Regulates polymerase II transcription. Also required for efficient transcription by RNA polymerase I, and more specifically the polymerase I transcription termination step. Regulates negatively DNA replication. Not only involved in transcription-related chromatin-remodeling, but also required to maintain a specific chromatin configuration across the genome. Is also associated with histone deacetylase (HDAC) activity (By similarity). Required for the bridging of SNF2, the FACT complex, the PAF complex as well as the U2 snRNP complex to H3K4me3. Functions to modulate the efficiency of pre-mRNA splicing in part through physical bridging of spliceosomal components to H3K4me3. Required for maintaining open chromatin and pluripotency in embryonic stem cells.
    • Sequence similarities

      Belongs to the SNF2/RAD54 helicase family.
      Contains 2 chromo domains.
      Contains 1 helicase ATP-binding domain.
      Contains 1 helicase C-terminal domain.
    • Domain

      The 2 chromodomains are involved in the binding to the histone H3 methyllysine at position 4 (H3K4me3).
    • Cellular localization

      Nucleus. Cytoplasm. Is released into the cytoplasm when cells enter mitosis and is reincorporated into chromatin during telophase-cytokinesis.
    • Information by UniProt
    • Database links

    • Alternative names

      • ATP dependent helicase CHD 1 antibody
      • ATP-dependent helicase CHD1 antibody
      • CHD 1 antibody
      • CHD-1 antibody
      • Chd1 antibody
      • CHD1_HUMAN antibody
      • Chromodomain helicase DNA binding protein 1 antibody
      • Chromodomain-helicase-DNA-binding protein 1 antibody
      • DKFZp686E2337 antibody
      • OTTHUMP00000222625 antibody
      see all

    Images

    • Chd1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab246009 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using an alternative anti-Chd1 antibody at 1 µg/ml.

      Lane 1: ab246009 IP in HeLa whole cell lysate.
      Lane 2: Control IgG IP in HeLa whole cell lysate.

      Detection: Chemiluminescence with exposure time of 10 seconds.

    References

    ab246009 has not yet been referenced specifically in any publications.

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