Overview

  • Product name

    Anti-CHD1L antibody [2170C3a]
    See all CHD1L primary antibodies
  • Description

    Mouse monoclonal [2170C3a] to CHD1L
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IP, Dot blot, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant human CHD1L fragment

  • Positive control

    • HEK293 whole cell lysate.
  • General notes

    This product was changed from ascites to tissue culture supernatant on 9th August 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    pH: 7.40
    Preservative: 0.05% Sodium azide
    Constituents: 1% BSA, 0.03% Potassium phosphate, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.0225% Potassium chloride, PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from TCS
  • Clonality

    Monoclonal
  • Clone number

    2170C3a
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab51324 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
Dot blot
IHC-P
  • Application notes
    Dot: Use at an assay dependent dilution.
    IHC-P: Use at an assay dependent dilution (PMID 20335658).
    IP: Use at 10µg/mg RIPA lysate of HeLa cells.
    WB: 1/50. Detects a band of approximately 101 kDa (predicted molecular weight: 101 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      DNA helicase which plays a role in chromatin-remodeling following DNA damage. Targeted to sites of DNA damage through interaction with poly(ADP-ribose) and functions to regulate chromatin during DNA repair. Able to catalyze nucleosome sliding in an ATP-dependent manner. Helicase activity is strongly stimulated upon poly(ADP-ribose)-binding.
    • Tissue specificity

      Frequently overexpressed in hepatomacellular carcinomas.
    • Sequence similarities

      Belongs to the SNF2/RAD54 helicase family.
      Contains 1 helicase ATP-binding domain.
      Contains 1 helicase C-terminal domain.
      Contains 1 Macro domain.
    • Domain

      The macro domain mediates non-covalent poly(ADP-ribose)-binding and recruitment to DNA damage sites.
    • Cellular localization

      Nucleus. Localizes at sites of DNA damage. Probably recruited to DNA damage sites by PARylated PARP1.
    • Information by UniProt
    • Database links

    • Alternative names

      • ALC1 antibody
      • Amplified in liver cancer 1 antibody
      • Amplified in liver cancer protein 1 antibody
      • chd1l antibody
      • CHD1L_HUMAN antibody
      • CHDL antibody
      • Chromodomain helicase DNA binding protein 1 like antibody
      • Chromodomain-helicase-DNA-binding protein 1-like antibody
      • FLJ22530 antibody
      see all

    Images

    • IHC image of ab51324 staining in human kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab51324, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • Anti-CHD1L antibody [2170C3a] (ab51324) at 1/50 dilution + HEK293 whole cell lysate at 25 µg

      Secondary
      anti-mouse IgG at 1/2500 dilution

      Developed using the ECL technique.

      Predicted band size: 101 kDa
      Observed band size: 101 kDa

    • Immunoprecipitation analysis using ab51324 at 5ug/500ug RIPA lysate of HeLa cells. Immunoblot uisng ab51324 at 1/50 dilution and 1/2500 anti-mouse IgG as secondary.

    References

    This product has been referenced in:

    See all 5 Publications for this product

    Customer reviews and Q&As

    Filter by Application

    Filter by Species

    Filter by Ratings

    1-2 of 2 Abreviews

    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Sample
    Human Cell lysate - whole cell (cancer cell lines)
    Specification
    cancer cell lines
    Blocking step
    cold fish geletin as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Jun 02 2014

    Application
    Western blot
    Loading amount
    25 µg
    Gel Running Conditions
    Reduced Denaturing (7.5)
    Sample
    Human Cell lysate - whole cell (Hela cells)
    Specification
    Hela cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

    Mr. Peter Rüthemann

    Verified customer

    Submitted Jul 04 2013

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up