Product nameAnti-CHD1L antibody [2170C3a]
See all CHD1L primary antibodies
DescriptionMouse monoclonal [2170C3a] to CHD1L
Tested applicationsSuitable for: WB, IP, Dot blot, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Recombinant human CHD1L fragment
- HEK293 whole cell lysate.
This product was changed from ascites to tissue culture supernatant on 9th August 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, 0.03% Potassium phosphate, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.0225% Potassium chloride, PBS
Concentration information loading...
PurityProtein G purified
Purification notesPurified from TCS
Our Abpromise guarantee covers the use of ab51324 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use at an assay dependent dilution (PMID 20335658).
IP: Use at 10µg/mg RIPA lysate of HeLa cells.
WB: 1/50. Detects a band of approximately 101 kDa (predicted molecular weight: 101 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionDNA helicase which plays a role in chromatin-remodeling following DNA damage. Targeted to sites of DNA damage through interaction with poly(ADP-ribose) and functions to regulate chromatin during DNA repair. Able to catalyze nucleosome sliding in an ATP-dependent manner. Helicase activity is strongly stimulated upon poly(ADP-ribose)-binding.
Tissue specificityFrequently overexpressed in hepatomacellular carcinomas.
Sequence similaritiesBelongs to the SNF2/RAD54 helicase family.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 1 Macro domain.
DomainThe macro domain mediates non-covalent poly(ADP-ribose)-binding and recruitment to DNA damage sites.
Cellular localizationNucleus. Localizes at sites of DNA damage. Probably recruited to DNA damage sites by PARylated PARP1.
- Information by UniProt
- ALC1 antibody
- Amplified in liver cancer 1 antibody
- Amplified in liver cancer protein 1 antibody
IHC image of ab51324 staining in human kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab51324, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Anti-CHD1L antibody [2170C3a] (ab51324) at 1/50 dilution + HEK293 whole cell lysate at 25 µg
anti-mouse IgG at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 101 kDa
Observed band size: 101 kDa
Immunoprecipitation analysis using ab51324 at 5ug/500ug RIPA lysate of HeLa cells. Immunoblot uisng ab51324 at 1/50 dilution and 1/2500 anti-mouse IgG as secondary.
This product has been referenced in:
- Tsuda M et al. ALC1/CHD1L, a chromatin-remodeling enzyme, is required for efficient base excision repair. PLoS One 12:e0188320 (2017). Read more (PubMed: 29149203) »
- Oza J et al. A Novel Role of Chromodomain Protein CBX8 in DNA Damage Response. J Biol Chem 291:22881-22893 (2016). Read more (PubMed: 27555324) »