Western blot abreview for Anti-CHD1L antibody [2170C3a]

Excellent
Abreviews
Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing (7.5)
Sample
Human Cell lysate - whole cell (Hela cells)
Specification
Hela cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/50

Secondary antibody

Name
Non-Abcam antibody was used: Sigma A2304
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/20000

Detection

Detection method
ECL
Bands
Specific: 100 kDa
Exposure
2 minute(s) and 26 second(s)
Negative control
Depletion of CHD1L by 3 different siRNA. The Knockdown has been performed for 3 days at a concentration of 16 nM.
Positive control
HeLa total cell lysate

Additional data

Additional Notes
If interested I can supply you with the siRNA sequence.

Mr. Peter Rüthemann

Verified customer

Submitted Jul 04 2013

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