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Gel Running Conditions
Reduced Denaturing (7.5)
Human Cell lysate - whole cell (Hela cells)
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
12 hour(s) and 0 minute(s) · Temperature: 4°C
Non-Abcam antibody was used: Sigma A2304
Host species: Goat
Conjugation: Horse Radish Peroxidase
2 minute(s) and 26 second(s)
Depletion of CHD1L by 3 different siRNA. The Knockdown has been performed for 3 days at a concentration of 16 nM.
HeLa total cell lysate
If interested I can supply you with the siRNA sequence.
Mr. Peter Rüthemann
Submitted Jul 04 2013