Western blot abreview for Anti-CHD1L antibody [2170C3a]

Excellent
Abreviews
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (4-12%)
Sample
Human Cell lysate - whole cell (cancer cell lines)
Specification
cancer cell lines
Blocking step
cold fish geletin as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2% cold fish geletin
Dilution
1/50

Secondary antibody

Name
Non-Abcam antibody was used: Horse anti mouse IgG
Host species: Horse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000

Detection

Detection method
ECL
Bands
Specific: 100 kDa
Exposure
1 minute(s) and 0 second(s)
Negative control
not included
Positive control
not included

Abcam user community

Verified customer

Submitted Jun 02 2014

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