Key features and details
- Rabbit polyclonal to CHD1L
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CHD1L antibody
See all CHD1L primary antibodies
DescriptionRabbit polyclonal to CHD1L
Tested applicationsSuitable for: IPmore details
Unsuitable for: WB
Species reactivityReacts with: Human
Synthetic peptide corresponding to a region within amino acids 847-897 of Human CHD1L (NP_004275.3).
- 293T whole cell lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab118799 was affinity purified using an epitope specific to CHD1L immobilized on solid support.
Our Abpromise guarantee covers the use of ab118799 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
FunctionDNA helicase which plays a role in chromatin-remodeling following DNA damage. Targeted to sites of DNA damage through interaction with poly(ADP-ribose) and functions to regulate chromatin during DNA repair. Able to catalyze nucleosome sliding in an ATP-dependent manner. Helicase activity is strongly stimulated upon poly(ADP-ribose)-binding.
Tissue specificityFrequently overexpressed in hepatomacellular carcinomas.
Sequence similaritiesBelongs to the SNF2/RAD54 helicase family.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 1 Macro domain.
DomainThe macro domain mediates non-covalent poly(ADP-ribose)-binding and recruitment to DNA damage sites.
Cellular localizationNucleus. Localizes at sites of DNA damage. Probably recruited to DNA damage sites by PARylated PARP1.
- Information by UniProt
- ALC1 antibody
- Amplified in liver cancer 1 antibody
- Amplified in liver cancer protein 1 antibody
Detection of CHD1L in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab118799 at 6 µg/mg lysate for IP (Lane 1). For WB detection an anti-CHD1L antibody which recognizes an upstream epitope was used at 1 µg/ml. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 10 seconds.
ab118799 has not yet been referenced specifically in any publications.