Overview

  • Product name

  • Description

    Rabbit polyclonal to CHD1L
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Cow
  • Immunogen

    Recombinant fragment within Human CHD1L (C terminal). The exact sequence is proprietary.
    Database link: Q86WJ1

  • Positive control

    • WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin
    Constituents: PBS, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab227608 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 101 kDa.

Target

  • Function

    DNA helicase which plays a role in chromatin-remodeling following DNA damage. Targeted to sites of DNA damage through interaction with poly(ADP-ribose) and functions to regulate chromatin during DNA repair. Able to catalyze nucleosome sliding in an ATP-dependent manner. Helicase activity is strongly stimulated upon poly(ADP-ribose)-binding.
  • Tissue specificity

    Frequently overexpressed in hepatomacellular carcinomas.
  • Sequence similarities

    Belongs to the SNF2/RAD54 helicase family.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
    Contains 1 Macro domain.
  • Domain

    The macro domain mediates non-covalent poly(ADP-ribose)-binding and recruitment to DNA damage sites.
  • Cellular localization

    Nucleus. Localizes at sites of DNA damage. Probably recruited to DNA damage sites by PARylated PARP1.
  • Information by UniProt
  • Database links

  • Alternative names

    • ALC1 antibody
    • Amplified in liver cancer 1 antibody
    • Amplified in liver cancer protein 1 antibody
    • chd1l antibody
    • CHD1L_HUMAN antibody
    • CHDL antibody
    • Chromodomain helicase DNA binding protein 1 like antibody
    • Chromodomain-helicase-DNA-binding protein 1-like antibody
    • FLJ22530 antibody
    see all

Images

  • All lanes : Anti-CHD1L antibody (ab227608) at 1/3000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 101 kDa



    7.5% SDS-PAGE

References

ab227608 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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