Key features and details
- Rabbit polyclonal to CHD1L
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CHD1L antibody
See all CHD1L primary antibodies
DescriptionRabbit polyclonal to CHD1L
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human CHD1L aa 704-897.
Database link: Q86WJ1
- WB: HepG2, HeLa and A549 whole cell lysate. ICC/IF: HepG2 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity greater than 95%.
Our Abpromise guarantee covers the use of ab237768 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000.|
|ICC/IF||1/200 - 1/500.|
FunctionDNA helicase which plays a role in chromatin-remodeling following DNA damage. Targeted to sites of DNA damage through interaction with poly(ADP-ribose) and functions to regulate chromatin during DNA repair. Able to catalyze nucleosome sliding in an ATP-dependent manner. Helicase activity is strongly stimulated upon poly(ADP-ribose)-binding.
Tissue specificityFrequently overexpressed in hepatomacellular carcinomas.
Sequence similaritiesBelongs to the SNF2/RAD54 helicase family.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 1 Macro domain.
DomainThe macro domain mediates non-covalent poly(ADP-ribose)-binding and recruitment to DNA damage sites.
Cellular localizationNucleus. Localizes at sites of DNA damage. Probably recruited to DNA damage sites by PARylated PARP1.
- Information by UniProt
- ALC1 antibody
- Amplified in liver cancer 1 antibody
- Amplified in liver cancer protein 1 antibody
All lanes : Anti-CHD1L antibody (ab237768) at 1/500 dilution
Lane 1 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : A549 (Human lung carcinoma cell line) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
HepG2 (human liver hepatocellular carcinoma cell line) cells stained for CHD1L (Green) using ab237768 at 1/200 dilution in ICC/IF, followed by an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody.
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the antibody overnight at 4°C. Counterstained with DAPI.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab237768 has not yet been referenced specifically in any publications.