Overview

  • Product name

  • Description

    Rabbit polyclonal to CHD1L
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human CHD1L aa 704-897.
    Database link: Q86WJ1

  • Positive control

    • WB: HepG2, HeLa and A549 whole cell lysate. ICC/IF: HepG2 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.03% Proclin
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purity greater than 95%.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab237768 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/5000.
ICC/IF 1/200 - 1/500.

Target

  • Function

    DNA helicase which plays a role in chromatin-remodeling following DNA damage. Targeted to sites of DNA damage through interaction with poly(ADP-ribose) and functions to regulate chromatin during DNA repair. Able to catalyze nucleosome sliding in an ATP-dependent manner. Helicase activity is strongly stimulated upon poly(ADP-ribose)-binding.
  • Tissue specificity

    Frequently overexpressed in hepatomacellular carcinomas.
  • Sequence similarities

    Belongs to the SNF2/RAD54 helicase family.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
    Contains 1 Macro domain.
  • Domain

    The macro domain mediates non-covalent poly(ADP-ribose)-binding and recruitment to DNA damage sites.
  • Cellular localization

    Nucleus. Localizes at sites of DNA damage. Probably recruited to DNA damage sites by PARylated PARP1.
  • Information by UniProt
  • Database links

  • Alternative names

    • ALC1 antibody
    • Amplified in liver cancer 1 antibody
    • Amplified in liver cancer protein 1 antibody
    • chd1l antibody
    • CHD1L_HUMAN antibody
    • CHDL antibody
    • Chromodomain helicase DNA binding protein 1 like antibody
    • Chromodomain-helicase-DNA-binding protein 1-like antibody
    • FLJ22530 antibody
    see all

Images

  • All lanes : Anti-CHD1L antibody (ab237768) at 1/500 dilution

    Lane 1 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 3 : A549 (Human lung carcinoma cell line) whole cell lysate

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
  • HepG2 (human liver hepatocellular carcinoma cell line) cells stained for CHD1L (Green) using ab237768 at 1/200 dilution in ICC/IF, followed by an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody.

    The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the antibody overnight at 4°C. Counterstained with DAPI.

References

ab237768 has not yet been referenced specifically in any publications.

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