Overview

  • Product name

  • Description

    Rabbit polyclonal to CHD2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Zebrafish
  • Immunogen

    Recombinant fragment corresponding to a region within N terminal amino acids 260-476 of Human CHD2.

  • Positive control

    • 293T and MOLT4 cell lysates; SW480 Xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab152139 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 57 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).

Target

  • Function

    Sequence-selective DNA-binding protein.
  • Sequence similarities

    Belongs to the SNF2/RAD54 helicase family.
    Contains 2 chromo domains.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2810013C04Rik antibody
    • 2810040A01Rik antibody
    • 5630401D06Rik antibody
    • AI851092 antibody
    • ATP dependent helicase CHD2 antibody
    • ATP-dependent helicase CHD2 antibody
    • BC029703 antibody
    • CHD 2 antibody
    • CHD-2 antibody
    • CHD2 antibody
    • CHD2_HUMAN antibody
    • chromodomain helicase dna binding protein antibody
    • Chromodomain-helicase-DNA-binding protein 2 antibody
    • DKFZp547I1315 antibody
    • DKFZp686E01200 antibody
    • DKFZP781D1727 antibody
    • EC 3.6.1.- antibody
    • EEOC antibody
    • FLJ38614 antibody
    see all

Images

  • Anti-CHD2 antibody (ab152139) at 1/1000 dilution + MOLT4 whole cell lysate at 30 µg

    Predicted band size: 57 kDa



    7.5% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded SW480 xenograft labeling CHD2 with ab152139 at 1/100 dilution.

References

ab152139 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

I have checked the immunogen used to raise both the anti-CHD2 antibody ab68301 and ab152139 in order to see if they would be likely to cross react with the Zebrafish CHD2 protein (Uniprot reference H0ZE34). From this, the immunogen of ab68301 matched with 95% homology whilst ab152139 matched with 88% homology. We would therefore expect both antibodies to cross react with the zebrafish protein. However, as we have not tested this ourselves we would not be able to guarantee this.
If you would like to purchase either of these antibodies and use it with your zebrafish samples you would be eligible to participate in the AbTrial scheme. This offer means that if you purchase ab68301 or ab15239 as normal, test the antibody with your samples and let us know of the results through an Abreview (no matter whether positive or negative) you would then be eligible to claim the value of the antibody tested off any product in our catalogue. More information on this offer can be found here:
www.abcam.com/abtrial
Please note that the antibody to be tested must be purchased, tested, the Abreview submitted and the free product claimed within a 4 month period.
If you would be interested in participating in this scheme please do let me know as a discount code needs to be issued prior to the purchase of ab68301 or ab15239.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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