Recombinant Anti-CHD3 antibody [EPNCIR110] (ab134125)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPNCIR110] to CHD3
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse
Related conjugates and formulations
Overview
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Product name
Anti-CHD3 antibody [EPNCIR110]
See all CHD3 primary antibodies -
Description
Rabbit monoclonal [EPNCIR110] to CHD3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide corresponding to Mouse CHD3.
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Positive control
- Mouse brain lysate, Raw264.7 cell lysate, NIH 3T3 cell lysate and cells, Mouse spleen tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of Gordon Hager. View antibodies from NCI Center for Cancer Research Collaboration.
Human, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPNCIR110 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab134125 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 260 kDa (predicted molecular weight: 234 kDa).
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/250.
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 260 kDa (predicted molecular weight: 234 kDa). |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/250. |
Target
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Function
Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Required for anchoring centrosomal pericentrin in both interphase and mitosis, for spindle organization and centrosome integrity. -
Tissue specificity
Widely expressed. -
Sequence similarities
Belongs to the SNF2/RAD54 helicase family.
Contains 2 chromo domains.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 2 PHD-type zinc fingers. -
Cellular localization
Nucleus. Cytoplasm > cytoskeleton > centrosome. Associates with centrosomes in interphase and mitosis. - Information by UniProt
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Database links
- Entrez Gene: 216848 Mouse
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Alternative names
- 226 antibody
- 2600010P09Rik antibody
- AF020312 antibody
see all
Images
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All lanes : Anti-CHD3 antibody [EPNCIR110] (ab134125) at 1/1000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : RAW 264.7 cell lysate
Lane 3 : NIH/3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 234 kDa
Observed band size: 260 kDa why is the actual band size different from the predicted? -
Immunohistochemical analysis of paraffin embedded Mouse spleen tissue labelling CHD3 with ab134125 at 1/100.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunofluorescent staining of 3T3 cells labelling CHD3 with ab134125 at 1/100.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab134125 has not yet been referenced specifically in any publications.