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    chd3-antibody-epncir110a-ab109195.pdf

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Immunology Immune System Diseases Autoimmune
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-CHD3 antibody [EPNCIR110A] (ab109195)

  • Datasheet
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Western blot - Anti-CHD3 antibody [EPNCIR110A] (ab109195)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHD3 antibody [EPNCIR110A] (ab109195)
  • Flow Cytometry - Anti-CHD3 antibody [EPNCIR110A] (ab109195)
  • Western blot - Anti-CHD3 antibody [EPNCIR110A] (ab109195)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHD3 antibody [EPNCIR110A] (ab109195)
  • Immunocytochemistry/ Immunofluorescence - Anti-CHD3 antibody [EPNCIR110A] (ab109195)
  • Anti-CHD3 antibody [EPNCIR110A] (ab109195)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPNCIR110A] to CHD3
  • Suitable for: Flow Cyt, WB, IHC-P, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-CHD3 antibody [EPNCIR110A]
    See all CHD3 primary antibodies
  • Description

    Rabbit monoclonal [EPNCIR110A] to CHD3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, WB, IHC-P, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Mouse CHD3 aa 1-100. The exact sequence is proprietary.
    Database link: B1AR17

  • Positive control

    • K562, HeLa, C6, and PC12 cell lysates, Human breast and Human ovary tissues; HeLa cells
  • General notes

    This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of Gordon Hager. View antibodies from NCI Center for Cancer Research Collaboration.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPNCIR110A
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Immune System Diseases
    • Autoimmune
    • Epigenetics and Nuclear Signaling
    • Chromatin Remodeling
    • Mi-2
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors

Associated products

  • Alternative Versions

    • Anti-CHD3 antibody [EPNCIR110A] - BSA and Azide free (ab240942)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa nuclear extract lysate (ab14655)
    • PC12 nuclear extract lysate (ab14884)

Applications

Our Abpromise guarantee covers the use of ab109195 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
WB 1/1000 - 1/10000. Detects a band of approximately 260 kDa (predicted molecular weight: 227 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat to 98°C, allow to cool for 10-20 minutes)
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Required for anchoring centrosomal pericentrin in both interphase and mitosis, for spindle organization and centrosome integrity.
    • Tissue specificity

      Widely expressed.
    • Sequence similarities

      Belongs to the SNF2/RAD54 helicase family.
      Contains 2 chromo domains.
      Contains 1 helicase ATP-binding domain.
      Contains 1 helicase C-terminal domain.
      Contains 2 PHD-type zinc fingers.
    • Cellular localization

      Nucleus. Cytoplasm > cytoskeleton > centrosome. Associates with centrosomes in interphase and mitosis.
    • Target information above from: UniProt accession Q12873 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 1107 Human
      • Entrez Gene: 216848 Mouse
      • Entrez Gene: 303241 Rat
      • Omim: 602120 Human
      • SwissProt: Q12873 Human
      • Unigene: 25601 Human
      • Unigene: 178246 Mouse
      • Unigene: 234402 Rat
      • Alternative names

        • 226 antibody
        • 2600010P09Rik antibody
        • AF020312 antibody
        • ATP dependent helicase CHD3 antibody
        • ATP-dependent helicase Chd3 antibody
        • CHD-3 antibody
        • Chd3 antibody
        • CHD3_HUMAN antibody
        • Chd7 antibody
        • Chromodomain-helicase-DNA-binding protein 3 antibody
        • hZFH antibody
        • MGC40857 antibody
        • Mi 2 autoantigen 240 kDa protein antibody
        • Mi 2a antibody
        • Mi-2 autoantigen 240 kDa protein antibody
        • Mi2 ALPHA antibody
        • Mi2-alpha antibody
        • Prp7 antibody
        • Prp9 1 antibody
        • ZFH antibody
        • Zinc finger helicase antibody
        • zinc-finger helicase (Snf2-like) antibody
        see all

      Images

      • Western blot - Anti-CHD3 antibody [EPNCIR110A] (ab109195)
        Western blot - Anti-CHD3 antibody [EPNCIR110A] (ab109195)

        Lane 1: Wild-type HAP1 cell lysate (20 µg)
        Lane 2: CHD3 knockout HAP1 cell lysate (20 µg)
        Lane 3: Raji cell lysate (20 µg)
        Lane 4: Y79 cell lysate (20 µg)
        Lanes 1 - 4: Merged signal (red and green). Green - ab109195 observed at 245 kDa. Red - loading control, ab8245, observed at 37 kDa.
        ab109195 was shown to specifically react with CHD3 when CHD3 knockout samples were used. Wild-type and CHD3 knockout samples were subjected to SDS-PAGE. ab109195 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW)preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHD3 antibody [EPNCIR110A] (ab109195)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHD3 antibody [EPNCIR110A] (ab109195)

        Immunohistochemical staining of paraffin-embedded Human breast tissue using ab109195 at a dilution of 1/100.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Flow Cytometry - Anti-CHD3 antibody [EPNCIR110A] (ab109195)
        Flow Cytometry - Anti-CHD3 antibody [EPNCIR110A] (ab109195)

        Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling CHD3 with purified ab109195 at 1/230 dilution(10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (ab150077)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black)(ab172730) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

      • Western blot - Anti-CHD3 antibody [EPNCIR110A] (ab109195)
        Western blot - Anti-CHD3 antibody [EPNCIR110A] (ab109195)
        All lanes : Anti-CHD3 antibody [EPNCIR110A] (ab109195) at 1/1000 dilution

        Lane 1 : K562 cell lysate
        Lane 2 : HeLa cell lysate
        Lane 3 : C6 cell lysate
        Lane 4 : PC12 cell lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 227 kDa
        Observed band size: 260 kDa
        why is the actual band size different from the predicted?

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHD3 antibody [EPNCIR110A] (ab109195)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHD3 antibody [EPNCIR110A] (ab109195)

        Immunohistochemical staining of paraffin-embedded Human ovary tissue using ab109195 at a dilution of 1/100.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunocytochemistry/ Immunofluorescence - Anti-CHD3 antibody [EPNCIR110A] (ab109195)
        Immunocytochemistry/ Immunofluorescence - Anti-CHD3 antibody [EPNCIR110A] (ab109195)
        Immunofluorescent staining of HeLa cells using ab109195 at a dilution of 1/100.
      • Anti-CHD3 antibody [EPNCIR110A] (ab109195)
        Anti-CHD3 antibody [EPNCIR110A] (ab109195)

      Protocols

      • Flow cytometry protocols
      • Immunohistochemistry protocols
      • Immunocytochemistry & immunofluorescence protocols
      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (1)

      Publishing research using ab109195? Please let us know so that we can cite the reference in this datasheet.

      ab109195 has been referenced in 1 publication.

      • Rodríguez-Castañeda F  et al. The SUMO protease SENP1 and the chromatin remodeler CHD3 interact and jointly affect chromatin accessibility and gene expression. J Biol Chem 293:15439-15454 (2018). PubMed: 30082317

      Customer reviews and Q&As

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