Recombinant Anti-CHD3 antibody [EPNCIR110A] (ab109195)
![Western blot - Anti-CHD3 antibody [EPNCIR110A] (ab109195)](https://www.abcam.com/ps/products/109/ab109195/Images/ab109195-256171-anti-chd3-antibody-epncir110a-western-blot.jpg "Western blot - Anti-CHD3 antibody [EPNCIR110A] (ab109195)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPNCIR110A] to CHD3
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Knockout validated
- Reacts with: Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-CHD3 antibody [EPNCIR110A]
See all CHD3 primary antibodies -
Description
Rabbit monoclonal [EPNCIR110A] to CHD3 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- K562, HeLa, C6, and PC12 cell lysates, Human breast and Human ovary tissues; HeLa cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of Gordon Hager. View antibodies from NCI Center for Cancer Research Collaboration.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPNCIR110A -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109195 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
Use at an assay dependent concentration.
|
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| WB |
1/1000 - 1/10000. Detects a band of approximately 260 kDa (predicted molecular weight: 227 kDa).
|
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat to 98°C, allow to cool for 10-20 minutes)
|
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| ICC/IF |
1/100 - 1/250.
|
| Notes |
|---|
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Flow Cyt (Intra)
Use at an assay dependent concentration. |
|
WB
1/1000 - 1/10000. Detects a band of approximately 260 kDa (predicted molecular weight: 227 kDa). |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat to 98°C, allow to cool for 10-20 minutes) |
|
ICC/IF
1/100 - 1/250. |
Target
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Function
Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Required for anchoring centrosomal pericentrin in both interphase and mitosis, for spindle organization and centrosome integrity. -
Tissue specificity
Widely expressed. -
Sequence similarities
Belongs to the SNF2/RAD54 helicase family.
Contains 2 chromo domains.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 2 PHD-type zinc fingers. -
Cellular localization
Nucleus. Cytoplasm > cytoskeleton > centrosome. Associates with centrosomes in interphase and mitosis. - Information by UniProt
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Database links
- Entrez Gene: 1107 Human
- Entrez Gene: 216848 Mouse
- Entrez Gene: 303241 Rat
- Omim: 602120 Human
- SwissProt: Q12873 Human
- Unigene: 25601 Human
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Alternative names
- 226 antibody
- 2600010P09Rik antibody
- AF020312 antibody
see all
Images
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Western blot - Anti-CHD3 antibody [EPNCIR110A] (ab109195)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: CHD3 knockout HAP1 cell lysate (20 µg)
Lane 3: Raji cell lysate (20 µg)
Lane 4: Y79 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab109195 observed at 245 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab109195 was shown to specifically react with CHD3 when CHD3 knockout samples were used. Wild-type and CHD3 knockout samples were subjected to SDS-PAGE. ab109195 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW)preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHD3 antibody [EPNCIR110A] (ab109195)](https://www.abcam.com/ps/products/109/ab109195/Images/ab109195-121813-anti-chd3-antibody-epncir110a-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHD3 antibody [EPNCIR110A] (ab109195)Immunohistochemical staining of paraffin-embedded Human breast tissue using ab109195 at a dilution of 1/100.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Flow Cytometry (Intracellular) - Anti-CHD3 antibody [EPNCIR110A] (ab109195)](https://www.abcam.com/ps/products/109/ab109195/Images/ab109195-276947-anti-chd3-antibody-epncir110a-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-CHD3 antibody [EPNCIR110A] (ab109195)Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling CHD3 with purified ab109195 at 1/230 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488) (ab150077) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) (ab172730) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
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![Western blot - Anti-CHD3 antibody [EPNCIR110A] (ab109195)](https://www.abcam.com/ps/products/109/ab109195/Images/ab109195-121814-anti-chd3-antibody-epncir110a-western-blot.jpg)
Western blot - Anti-CHD3 antibody [EPNCIR110A] (ab109195)All lanes : Anti-CHD3 antibody [EPNCIR110A] (ab109195) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : C6 cell lysate
Lane 4 : PC12 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 227 kDa
Observed band size: 260 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHD3 antibody [EPNCIR110A] (ab109195)](https://www.abcam.com/ps/products/109/ab109195/Images/ab109195-121812-anti-chd3-antibody-epncir110a-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHD3 antibody [EPNCIR110A] (ab109195)Immunohistochemical staining of paraffin-embedded Human ovary tissue using ab109195 at a dilution of 1/100.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-CHD3 antibody [EPNCIR110A] (ab109195)](https://www.abcam.com/ps/products/109/ab109195/Images/ab109195-121811-anti-chd3-antibody-epncir110a-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CHD3 antibody [EPNCIR110A] (ab109195)Immunofluorescent staining of HeLa cells using ab109195 at a dilution of 1/100. -
![Anti-CHD3 antibody [EPNCIR110A] (ab109195)](https://www.abcam.com/ps/products/109/ab109195/Images/ab109195-7-benefits-of-recombinant-antibodies.png)
Anti-CHD3 antibody [EPNCIR110A] (ab109195)
Protocols
Datasheets and documents
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Datasheet download
References (2)
ab109195 has been referenced in 2 publications.
- Xie J et al. The chromatin-remodeling enzyme CHD3 plays a role in embryonic viability but is dispensable for early vascular development. PLoS One 15:e0235799 (2020). PubMed: 32658897
- Rodríguez-Castañeda F et al. The SUMO protease SENP1 and the chromatin remodeler CHD3 interact and jointly affect chromatin accessibility and gene expression. J Biol Chem 293:15439-15454 (2018). PubMed: 30082317
