Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-CHD3 antibody [EPNCIR110A] - BSA and Azide free (ab240942)

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Immunocytochemistry/ Immunofluorescence - Anti-CHD3 antibody [EPNCIR110A] - BSA and Azide free (ab240942)
  • Western blot - Anti-CHD3 antibody [EPNCIR110A] - BSA and Azide free (ab240942)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHD3 antibody [EPNCIR110A] - BSA and Azide free (ab240942)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHD3 antibody [EPNCIR110A] - BSA and Azide free (ab240942)
  • Flow Cytometry - Anti-CHD3 antibody [EPNCIR110A] - BSA and Azide free (ab240942)
  • Anti-CHD3 antibody [EPNCIR110A] - BSA and Azide free (ab240942)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPNCIR110A] to CHD3 - BSA and Azide free
  • Suitable for: WB, Flow Cyt, ICC/IF, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-CHD3 antibody [EPNCIR110A] - BSA and Azide free
    See all CHD3 primary antibodies

  • Description

    Rabbit monoclonal [EPNCIR110A] to CHD3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IF, IHC-Pmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Mouse CHD3 aa 1-100. The exact sequence is proprietary.
    Database link: B1AR17

  • Positive control

    • WB: Wild Type HAP1 cell lysates. Raji and Y79 cell lysate. IHC-P: Human breast and ovary tissues. Flow Cyt: HeLa cells.

  • General notes

    ab240942 is the carrier-free version of ab109195 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab240942 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of Gordon Hager. View antibodies from NCI Center for Cancer Research Collaboration.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab240942 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 260 kDa (predicted molecular weight: 227 kDa).
Flow Cyt Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Heat to 98 degrees, allow to cool for 10-20 minutes.

 
  • Application notes
    Is unsuitable for IP.

    • Target

    • Function

      Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Required for anchoring centrosomal pericentrin in both interphase and mitosis, for spindle organization and centrosome integrity.

    • Tissue specificity

      Widely expressed.

    • Sequence similarities

      Belongs to the SNF2/RAD54 helicase family.
      Contains 2 chromo domains.
      Contains 1 helicase ATP-binding domain.
      Contains 1 helicase C-terminal domain.
      Contains 2 PHD-type zinc fingers.

    • Cellular localization

      Nucleus. Cytoplasm > cytoskeleton > centrosome. Associates with centrosomes in interphase and mitosis.
    • Information by UniProt

    • Database links

      • Alternative names

        • 226 antibody
        • 2600010P09Rik antibody
        • AF020312 antibody
        • ATP dependent helicase CHD3 antibody
        • ATP-dependent helicase Chd3 antibody
        • CHD-3 antibody
        • Chd3 antibody
        • CHD3_HUMAN antibody
        • Chd7 antibody
        • Chromodomain-helicase-DNA-binding protein 3 antibody
        • hZFH antibody
        • MGC40857 antibody
        • Mi 2 autoantigen 240 kDa protein antibody
        • Mi 2a antibody
        • Mi-2 autoantigen 240 kDa protein antibody
        • Mi2 ALPHA antibody
        • Mi2-alpha antibody
        • Prp7 antibody
        • Prp9 1 antibody
        • ZFH antibody
        • Zinc finger helicase antibody
        • zinc-finger helicase (Snf2-like) antibody
        see all

      Images

      • Immunocytochemistry/ Immunofluorescence - Anti-CHD3 antibody [EPNCIR110A] - BSA and Azide free (ab240942)
        Immunocytochemistry/ Immunofluorescence - Anti-CHD3 antibody [EPNCIR110A] - BSA and Azide free (ab240942)
        Immunofluorescent staining of HeLa cells using ab109195 at a dilution of 1/100.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109195)
      • Western blot - Anti-CHD3 antibody [EPNCIR110A] - BSA and Azide free (ab240942)
        Western blot - Anti-CHD3 antibody [EPNCIR110A] - BSA and Azide free (ab240942)

        Lane 1: Wild-type HAP1 cell lysate (20 µg)
        Lane 2: CHD3 knockout HAP1 cell lysate (20 µg)
        Lane 3: Raji cell lysate (20 µg)
        Lane 4: Y79 cell lysate (20 µg)
        Lanes 1 - 4: Merged signal (red and green). Green - ab109195 observed at 245 kDa. Red - loading control, ab8245, observed at 37 kDa.
        ab109195 was shown to specifically react with CHD3 when CHD3 knockout samples were used. Wild-type and CHD3 knockout samples were subjected to SDS-PAGE. ab109195 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW)preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab109195).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHD3 antibody [EPNCIR110A] - BSA and Azide free (ab240942)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHD3 antibody [EPNCIR110A] - BSA and Azide free (ab240942)

        Immunohistochemical staining of paraffin-embedded Human ovary tissue using ab109195 at a dilution of 1/100.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109195)

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHD3 antibody [EPNCIR110A] - BSA and Azide free (ab240942)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHD3 antibody [EPNCIR110A] - BSA and Azide free (ab240942)

        Immunohistochemical staining of paraffin-embedded Human breast tissue using ab109195 at a dilution of 1/100.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109195)

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Flow Cytometry - Anti-CHD3 antibody [EPNCIR110A] - BSA and Azide free (ab240942)
        Flow Cytometry - Anti-CHD3 antibody [EPNCIR110A] - BSA and Azide free (ab240942)

        Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling CHD3 with purified ab109195 at 1/230 dilution(10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (ab150077)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black)(ab172730) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109195)
      • Anti-CHD3 antibody [EPNCIR110A] - BSA and Azide free (ab240942)
        Anti-CHD3 antibody [EPNCIR110A] - BSA and Azide free (ab240942)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References (0)

      Publishing research using ab240942? Please let us know so that we can cite the reference in this datasheet.

      ab240942 has not yet been referenced specifically in any publications.

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