Anti-CHD4 antibody [3F2/4] - ChIP Grade (ab70469)

Mouse monoclonal CHD4 antibody [3F2/4]. Validated in WB, IP, ELISA, IHC, Flow Cyt, ChIP, ICC/IF and tested in Mouse, Human. Cited in 21 publication(s). Independently reviewed in 5 review(s).

Overview

  • Product name

    Anti-CHD4 antibody [3F2/4] - ChIP Grade
    See all CHD4 primary antibodies
  • Description

    Mouse monoclonal [3F2/4] to CHD4 - ChIP Grade
  • Host species

    Mouse
  • Tested applications

    Suitable for: ICC/IF, ChIP, ELISA, IP, IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human CHD4.

  • Positive control

    • IHC: Human breast carcinoma tissue.
  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Applications

Our Abpromise guarantee covers the use of ab70469 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
ChIP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml.
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones.
  • Sequence similarities

    Belongs to the SNF2/RAD54 helicase family.
    Contains 2 chromo domains.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
    Contains 2 PHD-type zinc fingers.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus. Cytoplasm > cytoskeleton > centrosome. Associates with centrosomes in interphase.
  • Information by UniProt
  • Database links

  • Alternative names

    • ATP dependent helicase CHD4 antibody
    • ATP-dependent helicase CHD4 antibody
    • CHD 4 antibody
    • CHD-4 antibody
    • CHD4 antibody
    • CHD4_HUMAN antibody
    • Chromodomain helicase DNA binding protein 4 antibody
    • Chromodomain-helicase-DNA-binding protein 4 antibody
    • Mi 2 autoantigen 218 kDa protein antibody
    • Mi 2b antibody
    • Mi-2 autoantigen 218 kDa protein antibody
    • Mi2 beta antibody
    • Mi2-beta antibody
    see all

Images

  • Immunocytochemical analysis of blastocysts from Wildtype and CHD4 -/- (Knockout) mice, labeling CHD4 with ab70469. Embryos were fixed in 2.5% paraformaldehyde, permeablised with 0.25% Triton X-100 and 3% polyvinlypyrrolidone in PBS, then blocked in 10% foetal calf serum and 0.1 Triton X-100 in PBS. Immunostaining with ab70469 (diluted 1/10,000) was performed overnight at 4°C. Staining with secondary antibody was for 1 hour at RT. DAPI used for nuclear staining (blue).

  • IHC image of CHD4 staining in human breast carcinoma formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab70469, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • CHD4 immunoprecipitation from murine brain tissue lysate using ab70469. 5000 µg of cell lysate was incubated with primary antibody (undiluted) and matrix (protein G) and incubated for 14 hours.

    An HRP-conjugated rabbit anti-CHD4 polyclonal (1/1000) was used to detect CHD4 immunoprecipitation.

    See Abreview

References

This product has been referenced in:

  • Hirota A  et al. The nucleosome remodeling and deacetylase complex protein CHD4 regulates neural differentiation of mouse embryonic stem cells by down-regulating p53. J Biol Chem 294:195-209 (2019). Read more (PubMed: 30409903) »
  • Biswas M  et al. MBD3/NuRD loss participates with KDM6A program to promote DOCK5/8 expression and Rac GTPase activation in human acute myeloid leukemia. FASEB J 33:5268-5286 (2019). Read more (PubMed: 30668141) »
See all 26 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (NIH-3T3 cells)
Permeabilization
Yes - tween-20
Specification
NIH-3T3 cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Aug 31 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (mouse urinary bladder)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH 6.0 citric acid
Permeabilization
Yes - tween-20
Specification
mouse urinary bladder
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jun 24 2015

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
Sample
Mouse Cell (MEL)
Specification
MEL
Permeabilization
No
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Dec 16 2013

Application
Western blot
Sample
Human Cell lysate - whole cell (293T cell)
Loading amount
30 µg
Specification
293T cell
Gel Running Conditions
Reduced Denaturing (7.5% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Min Young Kim

Verified customer

Submitted Dec 31 2012

Application
Immunoprecipitation
Sample
Mouse Tissue lysate - whole (brain)
Total protein in input
500 µg
Specification
brain
Immuno-precipitation step
Protein G

Dr. Catia Andreassi

Verified customer

Submitted Jul 16 2012

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