Product nameAnti-CHD4 antibody [EPR12229]
See all CHD4 primary antibodies
DescriptionRabbit monoclonal [EPR12229] to CHD4
The immunogen used for this product shares 95% homology with CDH3. Cross-reactivity with this protein has not been confirmed experimentally.
Tested applicationsSuitable for: ICC/IF, Flow Cyt, WBmore details
Unsuitable for: IHC-P or IP
Species reactivityReacts with: Human
Synthetic peptide within Human CHD4 aa 1800-1900. The exact sequence is proprietary.
Database link: Q14839
- NCCIT, 293T, HeLa and Jurkat whole cell lysate (ab7899); HeLa cells; Permeabilized 293T cells.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab181370 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50 - 1/100.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|WB||1/10000 - 1/50000. Detects a band of approximately 260 kDa (predicted molecular weight: 218 kDa).|
FunctionComponent of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones.
Sequence similaritiesBelongs to the SNF2/RAD54 helicase family.
Contains 2 chromo domains.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 2 PHD-type zinc fingers.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus. Cytoplasm > cytoskeleton > centrosome. Associates with centrosomes in interphase.
- Information by UniProt
- ATP dependent helicase CHD4 antibody
- ATP-dependent helicase CHD4 antibody
- CHD 4 antibody
All lanes : Anti-CHD4 antibody [EPR12229] (ab181370) at 1/10000 dilution
Lane 1 : NCCIT cell lysates
Lane 2 : 293T cell lysates
Lane 3 : HeLa cell lysates
Lane 4 : Jurkat cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 218 kDa
Immunofluorescent analysis of HeLa cells labeling CHD4 with ab181370 at 1/50 dilution.
Flow cytometric analysis of permeabilized 293T cells labeling CHD4 with ab181370 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
ab181370 has not yet been referenced specifically in any publications.