Overview

  • Product name

    Anti-CHD4 antibody [EPR12229]
    See all CHD4 primary antibodies
  • Description

    Rabbit monoclonal [EPR12229] to CHD4
  • Host species

    Rabbit
  • Specificity

    The immunogen used for this product shares 95% homology with CDH3. Cross-reactivity with this protein has not been confirmed experimentally.

  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, WBmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CHD4 aa 1800-1900. The exact sequence is proprietary.
    Database link: Q14839

  • Positive control

    • NCCIT, 293T, HeLa and Jurkat whole cell lysate (ab7899); HeLa cells; Permeabilized 293T cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab181370 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

WB 1/10000 - 1/50000. Detects a band of approximately 260 kDa (predicted molecular weight: 218 kDa).
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones.
    • Sequence similarities

      Belongs to the SNF2/RAD54 helicase family.
      Contains 2 chromo domains.
      Contains 1 helicase ATP-binding domain.
      Contains 1 helicase C-terminal domain.
      Contains 2 PHD-type zinc fingers.
    • Post-translational
      modifications

      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization

      Nucleus. Cytoplasm > cytoskeleton > centrosome. Associates with centrosomes in interphase.
    • Information by UniProt
    • Database links

    • Alternative names

      • ATP dependent helicase CHD4 antibody
      • ATP-dependent helicase CHD4 antibody
      • CHD 4 antibody
      • CHD-4 antibody
      • CHD4 antibody
      • CHD4_HUMAN antibody
      • Chromodomain helicase DNA binding protein 4 antibody
      • Chromodomain-helicase-DNA-binding protein 4 antibody
      • Mi 2 autoantigen 218 kDa protein antibody
      • Mi 2b antibody
      • Mi-2 autoantigen 218 kDa protein antibody
      • Mi2 beta antibody
      • Mi2-beta antibody
      see all

    Images

    • All lanes : Anti-CHD4 antibody [EPR12229] (ab181370) at 1/10000 dilution

      Lane 1 : NCCIT cell lysates
      Lane 2 : 293T cell lysates
      Lane 3 : HeLa cell lysates
      Lane 4 : Jurkat cell lysates

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 218 kDa

    • Immunofluorescent analysis of HeLa cells labeling CHD4 with ab181370 at 1/50 dilution.

    • Flow cytometric analysis of permeabilized 293T cells labeling CHD4 with ab181370 at 1/10 dilution (red) or a rabbit IgG (negative) (green).

    References

    ab181370 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab181370.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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