Anti-CHD6 antibody [2174C2a] (ab51330)

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Western blot - Anti-CHD6 antibody [2174C2a] (ab51330)

    Key features and details

    • Mouse monoclonal [2174C2a] to CHD6
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG1

    Overview

    • Product name

      Anti-CHD6 antibody [2174C2a]
      See all CHD6 primary antibodies

    • Description

      Mouse monoclonal [2174C2a] to CHD6

    • Host species

      Mouse

    • Tested applications

      Suitable for: WBmore details

    • Species reactivity

      Reacts with: Human

    • Immunogen

      Recombinant human CHD6 fragment

    • Positive control

      • HeLa whole cell lysate

    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.

    • Storage buffer

      pH: 7.40
      Preservative: 0.05% Sodium azide
      Constituents: 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.0225% Potassium chloride, 1% BSA, 0.03% Potassium phosphate, PBS
    • Concentration information loading...

    • Purity

      Protein G purified

    • Clonality

      Monoclonal

    • Clone number

      2174C2a

    • Isotype

      IgG1

    • Research areas

    Applications

    Our Abpromise guarantee covers the use of ab51330 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB 1/50. Predicted molecular weight: 305 kDa.

    Target

    • Function

      Probable transcription regulator.

    • Tissue specificity

      Ubiquitous.

    • Sequence similarities

      Belongs to the SNF2/RAD54 helicase family.
      Contains 2 chromo domains.
      Contains 1 helicase ATP-binding domain.
      Contains 1 helicase C-terminal domain.

    • Post-translational
      modifications

      Phosphorylated upon DNA damage, probably by ATM or ATR.

    • Cellular localization

      Nucleus.
    • Information by UniProt

    • Database links

      • Alternative names

        • ATP dependent helicase CHD6 antibody
        • ATP-dependent helicase CHD6 antibody
        • CHD-6 antibody
        • CHD5 antibody
        • CHD6 antibody
        • CHD6_HUMAN antibody
        • chromodomain helicase DNA binding protein 6 antibody
        • Chromodomain-helicase-DNA-binding protein 6 antibody
        • helicase C-terminal domain- and SNF2 N-terminal domain-containing protein antibody
        • KIAA1335 antibody
        • Radiation induced gene B protein antibody
        • Radiation-induced gene B protein antibody
        • RIGB antibody
        • RP4-620E11.2 antibody
        see all

      Images

      • Western blot - Anti-CHD6 antibody [2174C2a] (ab51330)
        Western blot - Anti-CHD6 antibody [2174C2a] (ab51330)
        Anti-CHD6 antibody [2174C2a] (ab51330) at 1/50 dilution + HeLa whole cell lysate at 25 µg

        Secondary
        anti-mouse IgG at 1/2500 dilution

        Developed using the ECL technique.

        Predicted band size: 305 kDa
        Observed band size: 305 kDa

      References (1)

      Publishing research using ab51330? Please let us know so that we can cite the reference in this datasheet.

      ab51330 has been referenced in 1 publication.

      • Alfonso R  et al. CHD6 chromatin remodeler is a negative modulator of influenza virus replication that relocates to inactive chromatin upon infection. Cell Microbiol 13:1894-906 (2011). PubMed: 21899694

      Customer reviews and Q&As

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      Western blot abreview for Anti-CHD6 antibody [2174C2a]

       Poor
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Cell lysate - nuclear (mouse and human cell lines)
      Gel Running Conditions
      Reduced Denaturing
      Loading amount
      20 µg
      Specification
      mouse and human cell lines
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
      Read More

      Abcam user community

      Verified customer

      Submitted Apr 20 2016

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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