Anti-CHD6 antibody (ab114095)
Key features and details
- Rabbit polyclonal to CHD6
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-CHD6 antibody
See all CHD6 primary antibodies -
Description
Rabbit polyclonal to CHD6 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Gorilla, Orangutan -
Immunogen
Synthetic peptide, corresponding to a region between amino acids 2665- 2715 of Human CHD6 (NP_115597.3).
-
Positive control
- Whole cell lysate from Hela cells.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH: 7 to 8 -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
ab114095 was affinity purified using an epitope specific to CHD6 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab114095 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/2000 - 1/10000. Predicted molecular weight: 305 kDa.
|
|
IP |
Use at 2-5 µg/mg of lysate.
|
Notes |
---|
WB
1/2000 - 1/10000. Predicted molecular weight: 305 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
Target
-
Function
Probable transcription regulator. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the SNF2/RAD54 helicase family.
Contains 2 chromo domains.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 84181 Human
- SwissProt: Q8TD26 Human
- Unigene: 371979 Human
-
Alternative names
- ATP dependent helicase CHD6 antibody
- ATP-dependent helicase CHD6 antibody
- CHD-6 antibody
see all
Images
-
All lanes : Anti-CHD6 antibody (ab114095) at 0.1 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Developed using the ECL technique.
Predicted band size: 305 kDa
Exposure time: 30 seconds -
ab114095, at 1 µg/ml, staining CHD6 in HeLa whole cell lysate immunoprecipitated using ab114095 at 3 µg/mg lysate (1 mg for IP; 20% of IP loaded/lane). Detection: Chemiluminescence with an exposure time of 10 seconds.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (2)
ab114095 has been referenced in 2 publications.
- Zhang B et al. The chromatin remodeler CHD6 promotes colorectal cancer development by regulating TMEM65-mediated mitochondrial dynamics via EGF and Wnt signaling. Cell Discov 8:130 (2022). PubMed: 36473865
- Xu G et al. Histone deacetylase 3 is associated with gastric cancer cell growth via the miR-454-mediated targeting of CHD5. Int J Mol Med 41:155-163 (2018). PubMed: 29115379