Overview

  • Product name
    Anti-CHD8 antibody
    See all CHD8 primary antibodies
  • Description
    Rabbit polyclonal to CHD8
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rabbit, Guinea pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 325-350 of Human CHD8 (NP_065971.2)

  • Positive control
    • HeLa cell lysate
  • General notes
    Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99.91% Tris citrate/phosphate
    Note: pH: 7-8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab114126 was affinity purified using an epitope specific to CHD8 immobilized on a solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab114126 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 291 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    DNA helicase that acts as a chromatin remodeling factor and regulates transcription. Acts as a transcription repressor by remodeling chromatin structure and recruiting histone H1 to target genes. Suppresses p53/TP53-mediated apoptosis by recruiting histone H1 and preventing p53/TP53 transactivation activity. Acts as a negative regulator of Wnt signaling pathway by regulating beta-catenin (CTNNB1) activity. Negatively regulates CTNNB1-targeted gene expression by being recruited specifically to the promoter regions of several CTNNB1 responsive genes. Involved in both enhancer blocking and epigenetic remodeling at chromatin boundary via its interaction with CTCF. Acts as a suppressor of STAT3 activity by suppressing the LIF-induced STAT3 transcriptional activity. Also acts as a transcription activator via its interaction with ZNF143 by participating to efficient U6 RNA polymerase III transcription.
  • Sequence similarities
    Belongs to the SNF2/RAD54 helicase family. CHD8 subfamily.
    Contains 2 chromo domains.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Post-translational
    modifications
    Sumoylated.
  • Cellular localization
    Nucleus. Localizes to the promoter regions of several CTNNB1-responsive genes. Also present at known CTCF target sites.
  • Information by UniProt
  • Database links
    • Alternative names
      • ATP dependent helicase CHD8 antibody
      • ATP-dependent helicase CHD8 antibody
      • Axis duplication inhibitor antibody
      • Beta catenin binding protein like antibody
      • CHD 8 antibody
      • CHD-8 antibody
      • chd8 antibody
      • CHD8_HUMAN antibody
      • Chromodomain helicase DNA binding protein 8 antibody
      • Chromodomain-helicase-DNA-binding protein 8 antibody
      • DKFZp686N17164 antibody
      • Duplin antibody
      • Helicase with SNF2 domain 1 antibody
      • HELSNF1 antibody
      • KIAA1564 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHD8 antibody (ab114126)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHD8 antibody (ab114126)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling CHD8 with ab114126 at 1/1000 (1µg/ml). Detection: DAB.
    • Western blot - Anti-CHD8 antibody (ab114126)
      Western blot - Anti-CHD8 antibody (ab114126)
      All lanes : Anti-CHD8 antibody (ab114126) at 0.1 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg

      Developed using the ECL technique.

      Predicted band size: 291 kDa


      Exposure time: 30 seconds
    • Immunoprecipitation - Anti-CHD8 antibody (ab114126)
      Immunoprecipitation - Anti-CHD8 antibody (ab114126)
      ab114126 at 3ug/mg of lysate detecting CHD8 by immunoprecipitation (lane 1). 1mg of HeLa whole cell lysate was used, 20% of immunoprecipitate was loaded. Lane 2: IgG control.
      Detection: chemiluminescence. Exposure time: 30 seconds.
    • Western blot - Anti-CHD8 antibody (ab114126)
      Western blot - Anti-CHD8 antibody (ab114126)
      All lanes : Anti-CHD8 antibody (ab114126) at 0.1 µg/ml

      Lane 1 : HeLa whole cell lysate
      Lane 2 : 293T whole cell lysate
      Lane 3 : Jurkat whole cell lysate
      Lane 4 : TCMK-1 whole cell lysate

      Lysates/proteins at 50 µg per lane.

      Developed using the ECL technique.

      Predicted band size: 291 kDa


      Exposure time: 3 minutes

    References

    This product has been referenced in:
    • Gompers AL  et al. Germline Chd8 haploinsufficiency alters brain development in mouse. Nat Neurosci 20:1062-1073 (2017). WB, CHIPseq ; Mouse . Read more (PubMed: 28671691) »
    • Cotney J  et al. The autism-associated chromatin modifier CHD8 regulates other autism risk genes during human neurodevelopment. Nat Commun 6:6404 (2015). IP, ChIP . Read more (PubMed: 25752243) »

    See all 2 Publications for this product

    Publishing research using ab114126? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    I can wait for that. Thanks.

    Thank you for your reply.

    I have gone ahead and processed the free of charge replacement, your new order number is * and we will ship the antibody to you as soon as we can.

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    Thank you for your reply.

    I just realized whilst I was trying to process the replacement that the ab57553, that it is currently out of stock, and we have an estimated delivery date of 24 July. Is this OK with you? I wanted to check in and ask ...

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    Thank you for your reply and for providing that information.

    I am sorry that the antibody was not working for you and based on the information that you have provided, I think the best course of action, is for me to either replace the antibody ...

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    First batch worked on a couple of Western blots and stopped working. Initially I thought the antibody got degraded by protease, etc. so I ordered another batch and never got Western blot working ever since. I'd like a replacement for another product (a...

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    Thank you for contacting Abcam.

    I am sorry about the issues you are having with ab114126 in western blot. The antibody is covered under our Abpromise for six months and is guaranteed to work in western blot on human samples . If we cannot reso...

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    What is the definition of ChIP grade?

    Thank you for calling Abcam. I just talked to a colleague and they informed me that when an antibody had been labeled ChIP grade, it means that Abcams in-house ChIP expert has reviewed all of the available ChIP data on the antibody and determined that ...

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