Key features and details
- Rabbit polyclonal to CHD8
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-CHD8 antibody
See all CHD8 primary antibodies
DescriptionRabbit polyclonal to CHD8
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human CHD8 aa 2252-2302. The exact sequence is proprietary. (NP_065971.2).
Database link: Q9HCK8-2
- IHC-P: Human ovarian carcinoma tissue. WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates. IP: HEK-293T whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab224830 was affinity purified using an epitope specific to CHD8 immobilized on solid support.
Our Abpromise guarantee covers the use of ab224830 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 290, 262 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionDNA helicase that acts as a chromatin remodeling factor and regulates transcription. Acts as a transcription repressor by remodeling chromatin structure and recruiting histone H1 to target genes. Suppresses p53/TP53-mediated apoptosis by recruiting histone H1 and preventing p53/TP53 transactivation activity. Acts as a negative regulator of Wnt signaling pathway by regulating beta-catenin (CTNNB1) activity. Negatively regulates CTNNB1-targeted gene expression by being recruited specifically to the promoter regions of several CTNNB1 responsive genes. Involved in both enhancer blocking and epigenetic remodeling at chromatin boundary via its interaction with CTCF. Acts as a suppressor of STAT3 activity by suppressing the LIF-induced STAT3 transcriptional activity. Also acts as a transcription activator via its interaction with ZNF143 by participating to efficient U6 RNA polymerase III transcription.
Sequence similaritiesBelongs to the SNF2/RAD54 helicase family. CHD8 subfamily.
Contains 2 chromo domains.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Cellular localizationNucleus. Localizes to the promoter regions of several CTNNB1-responsive genes. Also present at known CTCF target sites.
- Information by UniProt
- ATP dependent helicase CHD8 antibody
- ATP-dependent helicase CHD8 antibody
- Axis duplication inhibitor antibody
All lanes : Anti-CHD8 antibody (ab224830) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 290, 262 kDa
Exposure time: 3 minutes
Lysates prepared using NETN buffer.
Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for CHD8 using ab224830 at 1/200 dilution in immunohistochemical analysis. Detection: DAB staining.
The two panels show staining with different lots.
CHD8 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate prepared using NETN buffer with ab224830 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab224830 at 0.1 µg/ml.
Lane 1: ab224830 IP in HEK-293T whole cell lysate.
Lane 2: ab224830 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 minutes.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab224830 has not yet been referenced specifically in any publications.