Overview

  • Product name

  • Description

    Rabbit polyclonal to CHD8
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human CHD8 aa 2252-2302. The exact sequence is proprietary. (NP_065971.2).
    Database link: Q9HCK8-2

  • Positive control

    • IHC-P: Human ovarian carcinoma tissue. WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates. IP: HEK-293T whole cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab224830 was affinity purified using an epitope specific to CHD8 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab224830 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 290, 262 kDa.
IP Use at 2-10 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    DNA helicase that acts as a chromatin remodeling factor and regulates transcription. Acts as a transcription repressor by remodeling chromatin structure and recruiting histone H1 to target genes. Suppresses p53/TP53-mediated apoptosis by recruiting histone H1 and preventing p53/TP53 transactivation activity. Acts as a negative regulator of Wnt signaling pathway by regulating beta-catenin (CTNNB1) activity. Negatively regulates CTNNB1-targeted gene expression by being recruited specifically to the promoter regions of several CTNNB1 responsive genes. Involved in both enhancer blocking and epigenetic remodeling at chromatin boundary via its interaction with CTCF. Acts as a suppressor of STAT3 activity by suppressing the LIF-induced STAT3 transcriptional activity. Also acts as a transcription activator via its interaction with ZNF143 by participating to efficient U6 RNA polymerase III transcription.
  • Sequence similarities

    Belongs to the SNF2/RAD54 helicase family. CHD8 subfamily.
    Contains 2 chromo domains.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Post-translational
    modifications

    Sumoylated.
  • Cellular localization

    Nucleus. Localizes to the promoter regions of several CTNNB1-responsive genes. Also present at known CTCF target sites.
  • Information by UniProt
  • Database links

  • Alternative names

    • ATP dependent helicase CHD8 antibody
    • ATP-dependent helicase CHD8 antibody
    • Axis duplication inhibitor antibody
    • Beta catenin binding protein like antibody
    • CHD 8 antibody
    • CHD-8 antibody
    • chd8 antibody
    • CHD8_HUMAN antibody
    • Chromodomain helicase DNA binding protein 8 antibody
    • Chromodomain-helicase-DNA-binding protein 8 antibody
    • DKFZp686N17164 antibody
    • Duplin antibody
    • Helicase with SNF2 domain 1 antibody
    • HELSNF1 antibody
    • KIAA1564 antibody
    see all

Images

  • All lanes : Anti-CHD8 antibody (ab224830) at 0.1 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
    Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 290, 262 kDa


    Exposure time: 3 minutes


    Lysates prepared using NETN buffer.

  • Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for CHD8 using ab224830 at 1/200 dilution in immunohistochemical analysis. Detection: DAB staining.

    The two panels show staining with different lots.

  • CHD8 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate prepared using NETN buffer with ab224830 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab224830 at 0.1 µg/ml.

    Lane 1: ab224830 IP in HEK-293T whole cell lysate.

    Lane 2: ab224830 IP in HEK-293T whole cell lysate.

    Lane 2: Control IgG IP in HeLa whole cell lysate.

    Detection:  Chemiluminescence with exposure time of 3 minutes.

References

ab224830 has not yet been referenced specifically in any publications.

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