• Product name
  • Description
    Rabbit polyclonal to CHD8
  • Host species
  • Tested applications
    Suitable for: WB, IHC-P, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rabbit, Horse, Guinea pig, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to a region between residues 2252 and 2302 of Human CHD8 (NP_065971.2).

  • Positive control
    • Human Anaplastic Thyroid Carcinoma, Bladder Cell Carcinoma, Breast Carcinoma, Ewing Sarcoma, Non-Small Cell Lung Cancer, Ovarian Carcinoma, Pancreatic Islet Cell Tumor, Prostate Carcinoma, Small Cell Lung Cancer and Testicular Seminoma tissue. Mouse Colon Carcinoma CT26, Skin or Teratoma tissue.
  • General notes

    Concentration is optimized for IHC and not determined


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab84527 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 291 kDa. PubMed: 24705355
IHC-P 1/100 - 1/500.

Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining.

IP Use at an assay dependent concentration. PubMed: 23285124


  • Function
    DNA helicase that acts as a chromatin remodeling factor and regulates transcription. Acts as a transcription repressor by remodeling chromatin structure and recruiting histone H1 to target genes. Suppresses p53/TP53-mediated apoptosis by recruiting histone H1 and preventing p53/TP53 transactivation activity. Acts as a negative regulator of Wnt signaling pathway by regulating beta-catenin (CTNNB1) activity. Negatively regulates CTNNB1-targeted gene expression by being recruited specifically to the promoter regions of several CTNNB1 responsive genes. Involved in both enhancer blocking and epigenetic remodeling at chromatin boundary via its interaction with CTCF. Acts as a suppressor of STAT3 activity by suppressing the LIF-induced STAT3 transcriptional activity. Also acts as a transcription activator via its interaction with ZNF143 by participating to efficient U6 RNA polymerase III transcription.
  • Sequence similarities
    Belongs to the SNF2/RAD54 helicase family. CHD8 subfamily.
    Contains 2 chromo domains.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Post-translational
  • Cellular localization
    Nucleus. Localizes to the promoter regions of several CTNNB1-responsive genes. Also present at known CTCF target sites.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATP dependent helicase CHD8 antibody
    • ATP-dependent helicase CHD8 antibody
    • Axis duplication inhibitor antibody
    • Beta catenin binding protein like antibody
    • CHD 8 antibody
    • CHD-8 antibody
    • chd8 antibody
    • CHD8_HUMAN antibody
    • Chromodomain helicase DNA binding protein 8 antibody
    • Chromodomain-helicase-DNA-binding protein 8 antibody
    • DKFZp686N17164 antibody
    • Duplin antibody
    • Helicase with SNF2 domain 1 antibody
    • HELSNF1 antibody
    • KIAA1564 antibody
    see all


  • ab84527, at 1/100 dilution, staining CHD8 in formalin-fixed, paraffin-embedded human breast carcinoma by Immunohistochemistry, using DAB staining.
  • ab84527, at 1/100 dilution, staining CHD8 in formalin-fixed, paraffin-embedded human prostate carcinoma by Immunohistochemistry using DAB staining.
  • ab84527, at 1/100 dilution, staining CHD8 in formalin-fixed, paraffin-embedded mouse teratoma by Immunohistochemistry using DAB staining.


This product has been referenced in:
  • Tahara T  et al. Colorectal carcinomas with CpG island methylator phenotype 1 frequently contain mutations in chromatin regulators. Gastroenterology 146:530-538.e5 (2014). IHC-P ; Human . Read more (PubMed: 24211491) »
  • Schulz Y  et al. CHARGE and Kabuki syndromes: a phenotypic and molecular link. Hum Mol Genet N/A:N/A (2014). WB ; Human . Read more (PubMed: 24705355) »

See all 3 Publications for this product

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