Overview

  • Product name

  • Description

    Rabbit polyclonal to CHD8
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rabbit, Horse, Guinea pig, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to a region between residues 2252 and 2302 of Human CHD8 (NP_065971.2).

  • Positive control

    • Human Anaplastic Thyroid Carcinoma, Bladder Cell Carcinoma, Breast Carcinoma, Ewing Sarcoma, Non-Small Cell Lung Cancer, Ovarian Carcinoma, Pancreatic Islet Cell Tumor, Prostate Carcinoma, Small Cell Lung Cancer and Testicular Seminoma tissue. Mouse Colon Carcinoma CT26, Skin or Teratoma tissue.
  • General notes

    Concentration is optimized for IHC and not determined

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab84527 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 291 kDa. PubMed: 24705355
IHC-P 1/100 - 1/500.

Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining.

IP Use at an assay dependent concentration. PubMed: 23285124

Target

  • Function

    DNA helicase that acts as a chromatin remodeling factor and regulates transcription. Acts as a transcription repressor by remodeling chromatin structure and recruiting histone H1 to target genes. Suppresses p53/TP53-mediated apoptosis by recruiting histone H1 and preventing p53/TP53 transactivation activity. Acts as a negative regulator of Wnt signaling pathway by regulating beta-catenin (CTNNB1) activity. Negatively regulates CTNNB1-targeted gene expression by being recruited specifically to the promoter regions of several CTNNB1 responsive genes. Involved in both enhancer blocking and epigenetic remodeling at chromatin boundary via its interaction with CTCF. Acts as a suppressor of STAT3 activity by suppressing the LIF-induced STAT3 transcriptional activity. Also acts as a transcription activator via its interaction with ZNF143 by participating to efficient U6 RNA polymerase III transcription.
  • Sequence similarities

    Belongs to the SNF2/RAD54 helicase family. CHD8 subfamily.
    Contains 2 chromo domains.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Post-translational
    modifications

    Sumoylated.
  • Cellular localization

    Nucleus. Localizes to the promoter regions of several CTNNB1-responsive genes. Also present at known CTCF target sites.
  • Information by UniProt
  • Database links

  • Alternative names

    • ATP dependent helicase CHD8 antibody
    • ATP-dependent helicase CHD8 antibody
    • Axis duplication inhibitor antibody
    • Beta catenin binding protein like antibody
    • CHD 8 antibody
    • CHD-8 antibody
    • chd8 antibody
    • CHD8_HUMAN antibody
    • Chromodomain helicase DNA binding protein 8 antibody
    • Chromodomain-helicase-DNA-binding protein 8 antibody
    • DKFZp686N17164 antibody
    • Duplin antibody
    • Helicase with SNF2 domain 1 antibody
    • HELSNF1 antibody
    • KIAA1564 antibody
    see all

Images

  • ab84527, at 1/100 dilution, staining CHD8 in formalin-fixed, paraffin-embedded human breast carcinoma by Immunohistochemistry, using DAB staining.
  • ab84527, at 1/100 dilution, staining CHD8 in formalin-fixed, paraffin-embedded human prostate carcinoma by Immunohistochemistry using DAB staining.
  • ab84527, at 1/100 dilution, staining CHD8 in formalin-fixed, paraffin-embedded mouse teratoma by Immunohistochemistry using DAB staining.

References

This product has been referenced in:

  • Zhao C  et al. Dual Requirement of CHD8 for Chromatin Landscape Establishment and Histone Methyltransferase Recruitment to Promote CNS Myelination and Repair. Dev Cell 45:753-768.e8 (2018). Read more (PubMed: 29920279) »
  • Akhade VS  et al. Mechanism of Wnt signaling induced down regulation of mrhl long non-coding RNA in mouse spermatogonial cells. Nucleic Acids Res 44:387-401 (2016). Read more (PubMed: 26446991) »
See all 5 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Embryonic lung bud)
Permeabilization
No
Specification
Embryonic lung bud
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 24°C
Fixative
Paraformaldehyde

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Verified customer

Submitted Oct 04 2018

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