Chemiluminescent HRP Substrate (membrane) (ab5801)
Overview
-
Product name
Chemiluminescent HRP Substrate (membrane) -
Product overview
A 2 component chemiluminescent substrate for the detection of horseradish peroxidase (HRP) in western blotting.
The Abcam Chemiluminescent HRP Substrate (membrane) is very sensitive and great care must be taken to optimize the individual assay components (antibodies, conjugates, solid phase, etc.) to minimize background reactivity associated with nonspecific immunochemical reactivities
-
Notes
Prepare 1x substrate by mixing 1 ml of the chemiluminescent reagent with 2 ml of the chemiluminescent reaction buffer. Mix well and protect from light. More or less of the 1x substrate can be prepared using the same ratio.
Stability: Stable for a minimum of 18 months when stored at 2-8ºC in the original container and protected from light. The 1x diluted reagent is stable for several hours at room temperature when protected from light.
-
Tested applications
Suitable for: WBmore details
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 kit luminol reagent. 1 x 20ml reaction buffer. 2 x 20ml -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab5801 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use at an assay dependent concentration.
Incubate membrane in chemiluminescent substrate for 1 minute then rinse off. |
Notes |
---|
WB
Use at an assay dependent concentration. Incubate membrane in chemiluminescent substrate for 1 minute then rinse off. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (2)
ab5801 has been referenced in 2 publications.
- Wang P et al. Decorin reduces hypertrophic scarring through inhibition of the TGF-ß1/Smad signaling pathway in a rat osteomyelitis model. Exp Ther Med 12:2102-2108 (2016). WB . PubMed: 27698699
- Pacini S et al. Constitutive expression of pluripotency-associated genes in mesodermal progenitor cells (MPCs). PLoS One 5:e9861 (2010). PubMed: 20360837