Chemiluminescent HRP Substrate (membrane) (ab5801)


  • Product name
    Chemiluminescent HRP Substrate (membrane)
  • Product overview

    A 2 component chemiluminescent substrate for the detection of horseradish peroxidase (HRP) in western blotting.


    The Abcam Chemiluminescent HRP Substrate (membrane) is very sensitive and great care must be taken to optimize the individual assay components (antibodies, conjugates, solid phase, etc.) to minimize background reactivity associated with nonspecific immunochemical reactivities

  • Notes

    Prepare 1x substrate by mixing 1 ml of the chemiluminescent reagent with 2 ml of the chemiluminescent reaction buffer. Mix well and protect from light. More or less of the 1x substrate can be prepared using the same ratio.

    Stability: Stable for a minimum of 18 months when stored at 2-8ºC in the original container and protected from light. The 1x diluted reagent is stable for several hours at room temperature when protected from light.

  • Tested applications
    Suitable for: WBmore details



Our Abpromise guarantee covers the use of ab5801 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.

Incubate membrane in chemiluminescent substrate for 1 minute then rinse off.


This product has been referenced in:
  • Wang P  et al. Decorin reduces hypertrophic scarring through inhibition of the TGF-ß1/Smad signaling pathway in a rat osteomyelitis model. Exp Ther Med 12:2102-2108 (2016). WB . Read more (PubMed: 27698699) »
  • Pacini S  et al. Constitutive expression of pluripotency-associated genes in mesodermal progenitor cells (MPCs). PLoS One 5:e9861 (2010). Read more (PubMed: 20360837) »

See all 2 Publications for this product

Customer reviews and Q&As

Thank you for your enquiry. I can confirm that both products (ab5801 and ab65623) can be used for Western blot application, however ab65623 can be applied for more test (50). Your customer may be interested in our very new range of WB reagents (Opt...

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Thank you for calling Abcam. I have not been able to find a chemiluminescent EMSA kit in our catalogue. We do have several protein extraction kits though, please see the list below: Mammalian Cell Extraction Kit  ab65399 Plasma Membrane Protein Extract...

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Thank you for contacting us. We have two anti-M13 antibodies that may be of interest: ab6188 Anti-M13 + Fd Bacteriophage Coat Proteins antibody Binds specifically to phage coat proteins of M13 or fd phage. ...

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I can confirm that the chemiluminescent peroxidase substrate ab5801 is actually a bit more sensitive than the ECL or ECL+ products. The fact you are having low signal with this substrate and the antibodies ab6721 and ab12856 could be due to a numbe...

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