Key features and details
- Rabbit polyclonal to CHERP
- Suitable for: ICC/IF, IP, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-CHERP antibody
DescriptionRabbit polyclonal to CHERP
Tested applicationsSuitable for: ICC/IF, IP, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptides, post synthetically modified to achieve desired antigenicty.
- CHERP mRNA is expressed ubiquitously in various tissues including lung, brain, heart, pancreas, kidney and skeletal muscle.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferAntibody stabilization buffer.
Concentration information loading...
Purification notesAffinity purified against immobilised antigen.
Our Abpromise guarantee covers the use of ab15951 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500. Predicted molecular weight: 106 kDa.|
RelevanceThe Calcium homoeostasis endoplasmic reticulum protein (CHERP) gene has been recently identified and localized on chromosome 19p13.1. CHERP has high homology to an SR related CTD protein, which is known to interact with the largest subunit of RNA polymerase II. CHERP is an integral endoplasmic reticulum membrane protein is involved in calcium mobilization induced by thrombin. Confocal microscopy revealed that CHERP is associated with Ins(1,4,5)P3 receptor throughout the cytoplasm and perinuclear region in Jurkat T lymphocyte. CHERP Anti sense treatment induced a decrease in CHERP, which caused an impaired increase in free cytoplasmic calcium but calcium influx remains unaffected, with some deficient in endoplasmic reticulum calcium stores. In the CHERP depleted Jurkat T lymphocytes the calcium dependent translocation of nuclear factor of activated T cells (NFAT) from cytoplasm to nucleus was also suppressed with significant suppression of cell.
Cellular localizationCytoplasm. Cytoplasm; perinuclear region. Endoplasmic reticulum. Note: Distributed throughout the cytoplasm and also localizes to the perinuclear region of both human erythroleukemia (HEL) cells and Jurkat cells. Colocalizes with ITPR1.
- Calcium homeostasis endoplasmic reticulum protein antibody
- DAN16 antibody
- DAN26 antibody
Immunofluorescence analysis of HEK293 cells, staining CHERP with ab15951.
Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 1% BSA. Cells were incubated with primary antibody at 4°C overnight. A fluorescent-conjugated anti-rabbit IgG was used as a secondary antibody.
ab15951 has been referenced in 3 publications.
- Zhang D et al. Down-regulation of CHERP inhibits neuroblastoma cell proliferation and induces apoptosis through ER stress induction. Oncotarget 8:80956-80970 (2017). PubMed: 29113358
- Lin-Moshier Y et al. Re-evaluation of the role of calcium homeostasis endoplasmic reticulum protein (CHERP) in cellular calcium signaling. J Biol Chem 288:355-67 (2013). WB, ICC/IF . PubMed: 23148228
- Ryan T et al. Identification of Novel Ryanodine Receptor 1 (RyR1) Protein Interaction with Calcium Homeostasis Endoplasmic Reticulum Protein (CHERP). J Biol Chem 286:17060-8 (2011). WB ; Human . PubMed: 21454501