Overview

  • Product name
    Chicken Anti-Human IgG H&L (HRP)
    See all IgG secondary antibodies
  • Host species
    Chicken
  • Target species
    Human
  • Tested applications
    Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, ICC/IF, WBmore details
  • Immunogen

    human IgG whole molecule

  • Conjugation
    HRP

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.60
    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate
  • Concentration information loading...
  • Purity
    Affinity purified
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notes
    Horseradish Peroxidase (HRP)
  • Clonality
    Polyclonal
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6864 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot Use at an assay dependent concentration.
ELISA 1/30000.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Immunomicroscopy Use at an assay dependent concentration.
ICC/IF 1/500 - 1/2500.
WB 1/1000 - 1/5000.

References

ab6864 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for your reply. I am sorry we do not have any HRP secondary antibodies specific for the Fab fragments. We do have some that are raised against Fab2. I don't know if this will be helpful, but I have placed a link to the search results from our website below: https://www.abcam.com/index.html?pageconfig=searchresults&search=IgG&pt=7&sk=specif&sv=2314&sn=(Fab')2&l=2&fViewMore=1 I am sorry we do not have a product to meet your requirements on this occasion. If you have any further questions, please do not hesitate to contact us.

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Question
Answer

Thank you for your recent telephone enquiry. I can confirm that  ab6864  Chicken polyclonal Secondary Antibody to Human IgG is raised against the whole IgG molecule. As discussed on the telephone, as this is polyclonal it is likely to have some epitopes on the Fab fragment. I have checked with our source, and I am sorry that detection of the Fab fragments alone has not specifically been tested in this case. Therefore, we cannot guarantee there are epitopes on the Fab fragments.  I am sorry we do not have the data you require on this occasion. If you have any further questions, please do not hesitate to contact us.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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